Mateo José Juan
Departamento de Microbiología y Ecología, Universidad de Valencia, 46100 Burjassot, Spain.
Microorganisms. 2023 Apr 7;11(4):964. doi: 10.3390/microorganisms11040964.
A broad variety of microorganisms with useful characteristics in the field of biotechnology live on the surface of grapes; one of these microorganisms is . This yeast secretes a β-glucosidase that can be used in fermentative processes to liberate aromatic compounds. In this work, the synthesis of an exocellular β-glucosidase has been demonstrated and the optimal conditions to maximize the enzyme's effectiveness were determined. There was a maximum enzymatic activity at 28 °C and pH 4.5. Furthermore, the enzyme presents a great glucose and fructose tolerance, and to a lesser extent, ethanol tolerance. In addition, its activity was stimulated by calcium ions and low concentrations of ethanol and methanol. The impact of terpene content in wine was also determined. Because of these characteristics, β-glucosidase is a good candidate for use in enology.
葡萄表面生活着各种各样在生物技术领域具有有用特性的微生物；其中一种微生物是……这种酵母分泌一种β-葡萄糖苷酶，可用于发酵过程中释放芳香化合物。在这项工作中，已证明了胞外β-葡萄糖苷酶的合成，并确定了使该酶有效性最大化的最佳条件。在28℃和pH 4.5时酶活性最高。此外，该酶对葡萄糖和果糖具有很强的耐受性，对乙醇的耐受性稍弱。此外，其活性受到钙离子以及低浓度乙醇和甲醇的刺激。还确定了萜烯含量对葡萄酒的影响。由于这些特性，β-葡萄糖苷酶是酿酒学中使用的良好候选物。
