Park Min-Seon, Kim Yu-Jeong, Shin Han-Jae, Kwon Yoo Jin, Chu Jaeryang, Lee Inock, Kim Kyung Hwan, Kim Byoung Kook, Kim Seung-Hyung, Seo Hwi Won, Kim Tae-Won
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
College of Veterinary Medicine (BK21 FOUR Program), Chungnam National University, Daejeon 34131, Republic of Korea.
Microorganisms. 2023 Apr 7;11(4):967. doi: 10.3390/microorganisms11040967.
Probiotics have been shown to possess anti-inflammatory effects in the gut by directly reducing the production of pro-inflammatory cytokines and by secreting anti-inflammatory molecules. However, their systemic anti-inflammatory effects have not been thoroughly investigated. In this study, we aimed to develop probiotics that have efficacy in both intestinal and lung inflammation. KC3 (KC3), which was isolated from kimchi, was selected as a pre-candidate based on its inhibitory effects on the production of pro-inflammatory cytokines in vitro. To further validate the effectiveness of KC3, we used ear edema, DSS-induced colitis, and ambient particulate-matter-induced lung inflammation models. First, KC3 exhibited direct anti-inflammatory effects on intestinal cells with the inhibition of IL-1β and TNF-α production. Additionally, KC3 treatment alleviated ear edema and DSS-induced colic inflammation, improving colon length and increasing the number of regulatory T cells. Beyond its local intestinal anti-inflammatory activity, KC3 inhibited pro-inflammatory cytokines in the bronchoalveolar fluid and prevented neutrophil infiltration in the lungs. These results suggest that KC3 could be a potential functional ingredient with respiratory protective effects against air-pollutant-derived inflammation, as well as for the treatment of local gut disorders.
益生菌已被证明可通过直接减少促炎细胞因子的产生以及分泌抗炎分子，在肠道中发挥抗炎作用。然而，它们的全身抗炎作用尚未得到充分研究。在本研究中，我们旨在开发对肠道和肺部炎症均有效的益生菌。从泡菜中分离出的KC3，基于其在体外对促炎细胞因子产生的抑制作用，被选为候选菌株。为进一步验证KC3的有效性，我们使用了耳部水肿、葡聚糖硫酸钠（DSS）诱导的结肠炎以及环境颗粒物诱导的肺部炎症模型。首先，KC3对肠道细胞表现出直接抗炎作用，抑制白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的产生。此外，KC3治疗减轻了耳部水肿和DSS诱导的结肠炎症，改善了结肠长度并增加了调节性T细胞的数量。除了其局部肠道抗炎活性外，KC3还抑制支气管肺泡液中的促炎细胞因子，并防止肺部中性粒细胞浸润。这些结果表明，KC3可能是一种潜在的功能性成分，对空气污染物引起的炎症具有呼吸保护作用，也可用于治疗局部肠道疾病。
