在0℃、存在链烷醇铵十二烷基硫酸盐的条件下，阳离子对菠菜类囊体叶绿素蛋白在聚丙烯酰胺凝胶电泳上的分离有显著影响。
Marked effect of cations on the separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0 degree C in the presence of alkanolammonium dodecyl sulfates.
作者信息
Kubo K, Takagi T
出版信息
J Biochem. 1986 May;99(5):1545-8. doi: 10.1093/oxfordjournals.jbchem.a135626.
The separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0 degrees C in the presence of dodecyl sulfate is markedly influenced by the kind of surfactant cation in the media used for solubilization and electrophoresis. The mode of separation can thus be modulated through the cation selection. Three kinds of alkanolammonium dodecyl sulfates were tested and their abilities as to the dissociation of the molecular assemblies of chlorophyll-proteins were found to decrease in the following order: tris(hydroxymethyl)methylammonium, triethanolammonium and triisopropanolammonium. Comparison of the electrophoretic patterns obtained with different kinds of cation may help clarify the hierarchy of the molecular assemblies of chlorophyll-proteins.
在0摄氏度、存在十二烷基硫酸盐的条件下，于聚丙烯酰胺凝胶电泳上分离菠菜类囊体叶绿素蛋白时，用于溶解和电泳的介质中表面活性剂阳离子的种类会对其产生显著影响。因此，可通过阳离子选择来调节分离模式。测试了三种十二烷基硫酸链烷醇铵，发现它们使叶绿素蛋白分子聚集体解离的能力按以下顺序降低：三（羟甲基）甲基铵、三乙醇铵和三异丙醇铵。比较用不同种类阳离子获得的电泳图谱可能有助于阐明叶绿素蛋白分子聚集体的层级结构。
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