Manard Benjamin T, Bradley Veronica C, Quarles C Derrick, Hendriks Lyndsey, Dunlap Daniel R, Hexel Cole R, Sullivan Patrick, Andrews Hunter B
Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
Elemental Scientific, Inc., Omaha, NE 68122, USA.
Nanomaterials (Basel). 2023 Apr 9;13(8):1322. doi: 10.3390/nano13081322.
The work described herein assesses the ability to characterize gold nanoparticles (Au NPs) of 50 and 100 nm, as well as 60 nm silver shelled gold core nanospheres (Au/Ag NPs), for their mass, respective size, and isotopic composition in an automated and unattended fashion. Here, an innovative autosampler was employed to mix and transport the blanks, standards, and samples into a high-efficiency single particle (SP) introduction system for subsequent analysis by inductively coupled plasma-time of flight-mass spectrometry (ICP-TOF-MS). Optimized NP transport efficiency into the ICP-TOF-MS was determined to be >80%. This combination, SP-ICP-TOF-MS, allowed for high-throughput sample analysis. Specifically, 50 total samples (including blanks/standards) were analyzed over 8 h, to provide an accurate characterization of the NPs. This methodology was implemented over the course of 5 days to assess its long-term reproducibility. Impressively, the in-run and day-to-day variation of sample transport is assessed to be 3.54 and 9.52% relative standard deviation (%RSD), respectively. The determination of Au NP size and concentration was of <5% relative difference from the certified values over these time periods. Isotopic characterization of the Ag/Ag particles ( = 132,630) over the course of the measurements was determined to be 1.0788 ± 0.0030 with high accuracy (0.23% relative difference) when compared to the multi-collector-ICP-MS determination.
本文所述工作评估了以自动化和无人值守方式表征50纳米和100纳米金纳米颗粒（Au NPs）以及60纳米银壳金核纳米球（Au/Ag NPs）的质量、各自尺寸和同位素组成的能力。在此，采用了一种创新的自动进样器，将空白、标准品和样品混合并输送到高效单颗粒（SP）引入系统中，随后通过电感耦合等离子体飞行时间质谱（ICP-TOF-MS）进行分析。确定进入ICP-TOF-MS的纳米颗粒传输效率优化后大于80%。这种组合，即SP-ICP-TOF-MS，实现了高通量样品分析。具体而言，在8小时内分析了总共50个样品（包括空白/标准品），以准确表征纳米颗粒。该方法在5天内实施，以评估其长期重现性。令人印象深刻的是，样品传输的批内和日间变化评估相对标准偏差（%RSD）分别为3.54%和9.52%。在这些时间段内，Au纳米颗粒尺寸和浓度的测定与认证值的相对差异小于5%。与多接收器电感耦合等离子体质谱测定相比，在测量过程中对Ag/Ag颗粒（ = 132,630）的同位素表征确定为1.0788 ± 0.0030，具有高精度（相对差异0.23%）。
