感染牛白血病病毒的牛体内抗逆转录病毒限制因子和急性期蛋白的基因表达研究

Study of the Genetic Expression of Antiretroviral Restriction Factors and Acute Phase Proteins in Cattle Infected with Bovine Leukemia Virus.

作者信息

González-Méndez Ana S, Tórtora Pérez Jorge L, Rojas-Anaya Edith, Ramírez Álvarez Hugo

机构信息

Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education Cuautitlan, Veterinary Medical School, Campus 4, National Autonomous University of Mexico, Cuautitlan Izcalli, Mexico City CP 54714, Mexico.

Pacific Center Research Center, INIFAP, Guadalajara CP 44660, Mexico.

出版信息

Pathogens. 2023 Mar 29;12(4):529. doi: 10.3390/pathogens12040529.

DOI:10.3390/pathogens12040529

PMID:37111415

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10146972/

Abstract

The goal of this study was to analyze the genetic expression of antiretroviral restriction factors (ARF) and acute phase proteins (APP), as well as their correlation with proviral and viral loads in cattle with aleukemic (AL) and persistent lymphocytosis (PL). Complete blood samples were collected from a herd of dairy cows, and we extracted genetic material from peripheral blood leukocytes. Absolute quantification of the expression of ARF (APOBEC-Z1, Z2, and Z3; HEXIM-1, HEXIM-2, and BST2) and APP (haptoglobin (HP), and serum amyloid A (SAA)) was performed by qPCR. Statistical significance was observed in the expression of APOBEC-Z3 in BLV-infected animals. We only found positive correlations with a strong expression of the ARF genes in the AL group. The participation of APOBEC (Z1 and Z3), HEXIM-1, and HEXIM-2 was more frequently identified in BLV-infected animals. HEXIM-2 showed active gene expression in the AL group. Although the expression of ARF in early stages of infection (AL) maintains an important participation, in late stages (PL) it seems to have little relevance.

摘要

本研究的目的是分析抗逆转录病毒限制因子（ARF）和急性期蛋白（APP）的基因表达，以及它们与无白血病（AL）和持续性淋巴细胞增多症（PL）牛的前病毒和病毒载量的相关性。从一群奶牛中采集全血样本，并从外周血白细胞中提取遗传物质。通过qPCR对ARF（载脂蛋白B mRNA编辑酶催化多肽样蛋白Z1、Z2和Z3；六聚体模体蛋白1、六聚体模体蛋白2和骨髓基质抗原2）和APP（触珠蛋白（HP）和血清淀粉样蛋白A（SAA））的表达进行绝对定量。在BLV感染动物中观察到载脂蛋白B mRNA编辑酶催化多肽样蛋白Z3表达的统计学显著性。我们仅在AL组中发现ARF基因强表达存在正相关。在BLV感染动物中更频繁地发现载脂蛋白B mRNA编辑酶催化多肽样蛋白（Z1和Z3）、六聚体模体蛋白1和六聚体模体蛋白2的参与。六聚体模体蛋白2在AL组中显示出活跃的基因表达。尽管ARF在感染早期（AL）的表达保持重要作用，但在晚期（PL）似乎相关性较小。