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使用染料配体及相关吸附剂进行酶分离的策略。

Strategies for enzyme isolation using dye-ligand and related adsorbents.

作者信息

Scopes R K

出版信息

J Chromatogr. 1986 Apr 11;376:131-40. doi: 10.1016/s0378-4347(00)80830-0.

Abstract

Approaches to the use of a large range of dye-ligand and similar adsorbents for protein isolation are described. The adsorbents behave in a similar fashion relative to each other with a variety of different applied protein mixtures, so a system of dividing the adsorbents into five groups according to their protein-binding ability has been adopted. In this way it becomes unnecessary to screen all dyes, and an ideal selection of dyes and conditions can quickly be achieved.

摘要

本文描述了使用多种染料配体及类似吸附剂进行蛋白质分离的方法。在处理各种不同的蛋白质混合物时,这些吸附剂的行为方式彼此相似,因此采用了一种根据吸附剂的蛋白质结合能力将其分为五组的系统。这样一来,就无需对所有染料进行筛选,能够快速实现染料和条件的理想选择。

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