Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Poleczki 19, 02-822 Warsaw, Poland.
Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Sensors (Basel). 2023 Apr 13;23(8):3946. doi: 10.3390/s23083946.
Methods based on nucleic acid detection are currently the most commonly used technique in COVID-19 diagnostics. Although generally considered adequate, these methods are characterised by quite a long time-to-result and the necessity to prepare the material taken from the examined person-RNA isolation. For this reason, new detection methods are being sought, especially those characterised by the high speed of the analysis process from the moment of sampling to the result. Currently, serological methods of detecting antibodies against the virus in the patient's blood plasma have attracted much attention. Although they are less precise in determining the current infection, such methods shorten the analysis time to several minutes, making it possible to consider them a promising method for screening tests in people with suspected infection. The described study investigated the feasibility of a surface plasmon resonance (SPR)-based detection system for on-site COVID-19 diagnostics. A simple-to-use portable device was proposed for the fast detection of anti-SARS-CoV-2 antibodies in human plasma. SARS-CoV-2-positive and -negative patient blood plasma samples were investigated and compared with the ELISA test. The receptor-binding domain (RBD) of spike protein from SARS-CoV-2 was selected as a binding molecule for the study. Then, the process of antibody detection using this peptide was examined under laboratory conditions on a commercially available SPR device. The portable device was prepared and tested on plasma samples from humans. The results were compared with those obtained in the same patients using the reference diagnostic method. The detection system is effective in the detection of anti-SARS-CoV-2 with the detection limit of 40 ng/mL. It was shown that it is a portable device that can correctly examine human plasma samples within a 10 min timeframe.
方法基于核酸检测目前是最常用的技术在 COVID-19 诊断。虽然通常被认为是足够的,这些方法的特点是相当长的时间结果和准备材料从被检查的人——RNA 分离。出于这个原因,新的检测方法正在被寻求,尤其是那些特点是分析过程的速度高从取样到结果。目前,血清学方法检测病毒抗体在病人的血浆中引起了广泛关注。虽然他们不太精确在确定当前的感染,这样的方法缩短分析时间到几分钟,使得它可以考虑筛选测试的一个有前途的方法在有疑似感染的人。所描述的研究调查了基于表面等离子体共振 (SPR) 的检测系统的可行性现场 COVID-19 诊断。一个简单易用的便携式设备提出了快速检测抗 SARS-CoV-2 抗体在人类血浆。SARS-CoV-2 阳性和阴性患者的血浆样本进行了调查和比较与 ELISA 试验。受体结合域 (RBD) 刺突蛋白 SARS-CoV-2 被选为绑定分子的研究。然后,抗体检测过程使用这种肽检查在实验室条件下在商业上可用的 SPR 设备。便携式设备准备和测试在人类的血浆样本。结果与参考诊断方法在相同的患者中获得的结果进行了比较。检测系统是有效的检测 SARS-CoV-2 的检测限为 40ng/ml。结果表明,这是一个便携式设备可以正确地检查人类血浆样本在 10 分钟的时间内。
