吉露蜂蜜对聚苯乙烯微塑料对大鼠子宫形态、激素及性类固醇受体表达毒性作用的保护作用
Protective Role of Kelulut Honey against Toxicity Effects of Polystyrene Microplastics on Morphology, Hormones, and Sex Steroid Receptor Expression in the Uterus of Rats.
作者信息
Amran Nur Hanisah, Zaid Siti Sarah Mohamad, Meng Goh Yong, Salleh Annas, Mokhtar Mohd Helmy
机构信息
Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Department of Veterinary Pre-Clinical Science, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
出版信息
Toxics. 2023 Mar 29;11(4):324. doi: 10.3390/toxics11040324.
BACKGROUND
Microplastics (MPs) are an emerging global pollutant. Previous studies have revealed that chronic exposure to MPs can affect animal and human reproductive health, particularly by impairing the reproductive system's normal functions, which may increase the risk of infertility in both males and females. Kelulut honey (KH), an excellent source of antioxidants, has been used to counteract the disruptive effects of Polystyrene microplastics (PS-MPs) in the rat uterus. Thus, this study aimed to investigate the potential protective effects of Kelulut honey against PS-MPs-induced uterine toxicity in pubertal rats.
METHODS
Prepubertal female Sprague Dawley rats were divided into four groups (n = 8): (i) normal control group (NC: treated with deionized water), MPs-exposed group (M: exposed to PS-MPs at 2.5 mg/kg), (iii) Kelulut honey group (DM: pretreated with 1200 mg/kg of KH 30 minutes before they were administered with PS-MPs at 2.5 mg/kg), and (iv) Kelulut honey control group (DC: only treated with KH at 2.5 mg/kg). The rats were treated orally once daily for six consecutive weeks.
RESULTS
Uterine abnormalities in PS-MPs-exposed rats were significantly improved after concurrent treatment with Kelulut honey. Morphology improvement was observed and luminal epithelial cells seemed thicker with more goblet cells, glandular cells had a more regular and circular shape, stromal cell increased in size, interstitial gaps between stromal cells expanded, and the myometrium layer was thicker. Kelulut honey treatment also effectively normalized the suppressive effect of PS-MPs on the expression and distribution of sex steroid receptors (ERα and ERβ), as well as the level of serum gonadotropin (LH and FSH) and sex steroid (estradiol and progesterone) hormones.
CONCLUSION
Kelulut honey can protect the female reproductive system against the disruptive effects of PS-MPs. The phytochemical properties of Kelulut honey might be responsible for these beneficial benefits. However, future studies are warranted to identify the mechanisms involved.
背景
微塑料是一种新出现的全球污染物。先前的研究表明，长期接触微塑料会影响动物和人类的生殖健康，尤其是通过损害生殖系统的正常功能，这可能会增加男性和女性不孕的风险。吉露蜂蜜是一种优质的抗氧化剂来源，已被用于抵消聚苯乙烯微塑料对大鼠子宫的破坏作用。因此，本研究旨在探讨吉露蜂蜜对青春期大鼠聚苯乙烯微塑料诱导的子宫毒性的潜在保护作用。
方法
将青春期雌性斯普拉格-道利大鼠分为四组（n = 8）：（i）正常对照组（NC：用去离子水处理），（ii）微塑料暴露组（M：暴露于2.5 mg/kg的聚苯乙烯微塑料），（iii）吉露蜂蜜组（DM：在给予2.5 mg/kg聚苯乙烯微塑料前30分钟用1200 mg/kg的吉露蜂蜜预处理），以及（iv）吉露蜂蜜对照组（DC：仅用2.5 mg/kg的吉露蜂蜜处理）。大鼠连续六周每天口服给药一次。
结果
在与吉露蜂蜜同时治疗后，聚苯乙烯微塑料暴露大鼠的子宫异常得到显著改善。观察到形态学改善，管腔上皮细胞似乎更厚，杯状细胞更多，腺细胞形状更规则且呈圆形，基质细胞大小增加，基质细胞之间的间隙扩大，肌层更厚。吉露蜂蜜治疗还有效恢复了聚苯乙烯微塑料对性类固醇受体（雌激素受体α和雌激素受体β）的表达和分布、血清促性腺激素（促黄体生成素和促卵泡生成素）以及性类固醇（雌二醇和孕酮）激素水平的抑制作用。
结论
吉露蜂蜜可以保护雌性生殖系统免受聚苯乙烯微塑料的破坏作用。吉露蜂蜜的植物化学特性可能是这些有益作用的原因。然而，未来的研究有必要确定其中涉及的机制。
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