Kamal Datu Agasi Mohd, Ibrahim Siti Fatimah, Ugusman Azizah, Mokhtar Mohd Helmy
Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia.
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Malaysia Sabah, Kota Kinabalu 88400, Malaysia.
Antioxidants (Basel). 2022 Sep 22;11(10):1879. doi: 10.3390/antiox11101879.
Kelulut honey (KH) has been proven to have excellent antioxidative and anti-inflammatory properties with unique physicochemical characteristics. Therefore, we investigated the isolated and combined effects of KH, metformin, or clomiphene in alleviating oxidative stress and reproductive and metabolic abnormalities in polycystic ovary syndrome (PCOS). Female Sprague-Dawley (SD) rats were given 1 mg/kg/day of letrozole for 21 days to induce PCOS. PCOS rats were then divided into six treatment groups: untreated, metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were administered orally for 35 days. The physicochemical characteristics of KH were assessed through hydroxymethylfurfural, free acidity, diastase number, moisture content, sugar profile, metals, and mineral compounds. Additionally, we determined the semivolatile organic compounds present in KH through gas chromatography-mass spectrometry (GC/MS) analysis. KH and its combination with metformin or clomiphene were shown to improve the oestrus cycle, hormonal profile, and oxidative stress in PCOS rats. However, KH did not reduce the fasting blood glucose, insulin, and body weight gain in PCOS rats. These findings may provide a basis for future studies to discover the potential use of KH as a complementary treatment for women with PCOS.
吉露蜂蜂蜜（KH）已被证明具有出色的抗氧化和抗炎特性以及独特的物理化学特征。因此，我们研究了KH、二甲双胍或克罗米芬单独及联合使用对缓解多囊卵巢综合征（PCOS）氧化应激及生殖和代谢异常的作用。给雌性斯普拉格-道利（SD）大鼠每日腹腔注射1 mg/kg来曲唑，持续21天以诱导PCOS。然后将PCOS大鼠分为六个治疗组：未治疗组、二甲双胍（500 mg/kg/天）组、克罗米芬（2 mg/kg/天）组、KH（1 g/kg/天）组、KH（1 g/kg/天）与二甲双胍（500 mg/kg/天）联合组以及KH（1 g/kg/天）与克罗米芬（2 mg/kg/天）联合组。所有治疗均口服给药35天。通过羟甲基糠醛、游离酸度、淀粉酶值、水分含量、糖谱、金属和矿物质化合物评估KH的物理化学特征。此外，我们通过气相色谱-质谱（GC/MS）分析确定了KH中存在的半挥发性有机化合物。结果表明，KH及其与二甲双胍或克罗米芬的联合使用可改善PCOS大鼠的发情周期、激素水平和氧化应激。然而，KH并未降低PCOS大鼠的空腹血糖、胰岛素水平和体重增加。这些发现可能为未来研究探索KH作为PCOS女性辅助治疗的潜在用途提供依据。
