• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人巨细胞病毒衣壳组装蛋白前体（UL80.5）的 SUMOylation 影响其与主要衣壳蛋白（UL86）的相互作用和病毒复制。

The SUMOylation of Human Cytomegalovirus Capsid Assembly Protein Precursor (UL80.5) Affects Its Interaction with Major Capsid Protein (UL86) and Viral Replication.

机构信息

Basic Medical College, Hubei University of Chinese Medicine, Wuhan 430065, China.

Department of Biological Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

出版信息

Viruses. 2023 Apr 7;15(4):931. doi: 10.3390/v15040931.

DOI:10.3390/v15040931
PMID:37112911
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10145422/
Abstract

Human Cytomegalovirus Capsid Assembly Protein Precursor (pAP, UL80.5) plays a key role in capsid assembly by forming an internal protein scaffold with Major Capsid Protein (MCP, UL86) and other capsid subunits. In this study, we revealed UL80.5 as a novel SUMOylated viral protein. We confirmed that UL80.5 interacted with the SUMO E2 ligase UBC9 (58-93aa) and could be covalently modified by SUMO1/SUMO2/SUMO3 proteins. Lysine located within a ψKxE consensus motif on UL80.5 carboxy-terminal was the major SUMOylation site. Interestingly, the SUMOylation of UL80.5 restrained its interaction with UL86 but had no effects on translocating UL86 into the nucleus. Furthermore, we showed that the removal of the lysine SUMOylation site of UL80.5 inhibited viral replication. In conclusion, our data demonstrates that SUMOylation plays an important role in regulating UL80.5 functions and viral replication.

摘要

人巨细胞病毒衣壳组装蛋白前体 (pAP，UL80.5) 通过与主要衣壳蛋白 (MCP，UL86) 和其他衣壳亚基形成内部蛋白支架，在衣壳组装中发挥关键作用。在这项研究中，我们揭示了 UL80.5 是一种新型 SUMO 化病毒蛋白。我们证实 UL80.5 与 SUMO E2 连接酶 UBC9（58-93aa）相互作用，并可被 SUMO1/SUMO2/SUMO3 蛋白共价修饰。UL80.5 羧基末端 ψKxE 共有序列内的赖氨酸是主要的 SUMO 化位点。有趣的是，UL80.5 的 SUMO 化抑制了其与 UL86 的相互作用，但对 UL86 向核内转移没有影响。此外，我们表明 UL80.5 的赖氨酸 SUMO 化位点的缺失抑制了病毒复制。总之，我们的数据表明 SUMO 化在调节 UL80.5 功能和病毒复制中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/83ac8e1259b2/viruses-15-00931-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/de5d87027488/viruses-15-00931-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/f0cdd80c6155/viruses-15-00931-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/c58a2e6e06bd/viruses-15-00931-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/19f1c699b571/viruses-15-00931-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/58980c76623b/viruses-15-00931-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/83ac8e1259b2/viruses-15-00931-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/de5d87027488/viruses-15-00931-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/f0cdd80c6155/viruses-15-00931-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/c58a2e6e06bd/viruses-15-00931-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/19f1c699b571/viruses-15-00931-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/58980c76623b/viruses-15-00931-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe79/10145422/83ac8e1259b2/viruses-15-00931-g006.jpg

相似文献

1
The SUMOylation of Human Cytomegalovirus Capsid Assembly Protein Precursor (UL80.5) Affects Its Interaction with Major Capsid Protein (UL86) and Viral Replication.人巨细胞病毒衣壳组装蛋白前体（UL80.5）的 SUMOylation 影响其与主要衣壳蛋白（UL86）的相互作用和病毒复制。
Viruses. 2023 Apr 7;15(4):931. doi: 10.3390/v15040931.
2
Human cytomegalovirus capsid assembly protein precursor (pUL80.5) interacts with itself and with the major capsid protein (pUL86) through two different domains.人巨细胞病毒衣壳组装蛋白前体（pUL80.5）通过两个不同结构域与自身以及主要衣壳蛋白（pUL86）相互作用。
J Virol. 1997 Jan;71(1):179-90. doi: 10.1128/JVI.71.1.179-190.1997.
3
Nuclear localization sequences in cytomegalovirus capsid assembly proteins (UL80 proteins) are required for virus production: inactivating NLS1, NLS2, or both affects replication to strikingly different extents.巨细胞病毒衣壳装配蛋白（UL80蛋白）中的核定位序列是病毒产生所必需的：使核定位信号1（NLS1）、核定位信号2（NLS2）或两者失活对病毒复制的影响程度显著不同。
J Virol. 2008 Jun;82(11):5381-9. doi: 10.1128/JVI.02697-07. Epub 2008 Mar 19.
4
Assembly of herpes simplex virus capsids using the human cytomegalovirus scaffold protein: critical role of the C terminus.利用人巨细胞病毒支架蛋白组装单纯疱疹病毒衣壳：C末端的关键作用
J Virol. 1997 Feb;71(2):1281-91. doi: 10.1128/JVI.71.2.1281-1291.1997.
5
The human cytomegalovirus DNA polymerase processivity factor UL44 is modified by SUMO in a DNA-dependent manner.人巨细胞病毒 DNA 聚合酶延伸因子 UL44 以依赖 DNA 的方式被 SUMO 修饰。
PLoS One. 2012;7(11):e49630. doi: 10.1371/journal.pone.0049630. Epub 2012 Nov 15.
6
Sumoylation of the Carboxy-Terminal of Human Cytomegalovirus DNA Polymerase Processivity Factor UL44 Attenuates Viral DNA Replication.人巨细胞病毒DNA聚合酶持续合成因子UL44羧基末端的类泛素化修饰减弱病毒DNA复制。
Front Microbiol. 2021 Apr 21;12:652719. doi: 10.3389/fmicb.2021.652719. eCollection 2021.
7
The ND10 Component Promyelocytic Leukemia Protein Acts as an E3 Ligase for SUMOylation of the Major Immediate Early Protein IE1 of Human Cytomegalovirus.ND10 成分早幼粒细胞白血病蛋白作为人巨细胞病毒主要立即早期蛋白 IE1 进行 SUMO 化修饰的 E3 连接酶。
J Virol. 2017 Apr 28;91(10). doi: 10.1128/JVI.02335-16. Print 2017 May 15.
8
The amino-conserved domain of human cytomegalovirus UL80a proteins is required for key interactions during early stages of capsid formation and virus production.人巨细胞病毒UL80a蛋白的氨基保守结构域是衣壳形成早期关键相互作用和病毒产生所必需的。
J Virol. 2007 Jan;81(2):620-8. doi: 10.1128/JVI.01903-06. Epub 2006 Nov 1.
9
Site-specific SUMOylation of viral polymerase processivity factor: a way of localizingtoND10 subnuclear domains for restricted and self-controlled reproduction of herpesvirus.病毒聚合酶持续因子的位点特异性 SUMOylation：一种定位于 ND10 亚核域的方式，用于限制和自我控制疱疹病毒的复制。
Virulence. 2021 Dec;12(1):2883-2901. doi: 10.1080/21505594.2021.2000689.
10
The Human Cytomegalovirus IE1 Protein Antagonizes PML Nuclear Body-Mediated Intrinsic Immunity via the Inhibition of PML De Novo SUMOylation.人巨细胞病毒IE1蛋白通过抑制PML从头SUMO化拮抗PML核体介导的固有免疫。
J Virol. 2017 Jan 31;91(4). doi: 10.1128/JVI.02049-16. Print 2017 Feb 15.

本文引用的文献

1
Signalling mechanisms and cellular functions of SUMO.SUMO 的信号机制和细胞功能。
Nat Rev Mol Cell Biol. 2022 Nov;23(11):715-731. doi: 10.1038/s41580-022-00500-y. Epub 2022 Jun 24.
2
Insights in Post-Translational Modifications: Ubiquitin and SUMO.翻译后修饰的见解：泛素与小泛素样修饰物
Int J Mol Sci. 2022 Mar 18;23(6):3281. doi: 10.3390/ijms23063281.
3
SUMO-SIM interactions: From structure to biological functions.SUMO-SIM 相互作用：从结构到生物学功能。
Semin Cell Dev Biol. 2022 Dec;132:193-202. doi: 10.1016/j.semcdb.2021.11.007. Epub 2021 Nov 25.
4
Site-specific SUMOylation of viral polymerase processivity factor: a way of localizingtoND10 subnuclear domains for restricted and self-controlled reproduction of herpesvirus.病毒聚合酶持续因子的位点特异性 SUMOylation：一种定位于 ND10 亚核域的方式，用于限制和自我控制疱疹病毒的复制。
Virulence. 2021 Dec;12(1):2883-2901. doi: 10.1080/21505594.2021.2000689.
5
Human cytomegalovirus non-primary infection during pregnancy: antibody response, risk factors and newborn outcome.人巨细胞病毒妊娠非原发性感染：抗体应答、危险因素和新生儿结局。
Clin Microbiol Infect. 2022 Oct;28(10):1375-1381. doi: 10.1016/j.cmi.2021.09.013. Epub 2021 Sep 20.
6
Pathogenesis of human cytomegalovirus in the immunocompromised host.免疫功能低下宿主中人巨细胞病毒的发病机制。
Nat Rev Microbiol. 2021 Dec;19(12):759-773. doi: 10.1038/s41579-021-00582-z. Epub 2021 Jun 24.
7
Sumoylation of the Carboxy-Terminal of Human Cytomegalovirus DNA Polymerase Processivity Factor UL44 Attenuates Viral DNA Replication.人巨细胞病毒DNA聚合酶持续合成因子UL44羧基末端的类泛素化修饰减弱病毒DNA复制。
Front Microbiol. 2021 Apr 21;12:652719. doi: 10.3389/fmicb.2021.652719. eCollection 2021.
8
Structures and Divergent Mechanisms in Capsid Maturation and Stabilization Following Genome Packaging of Human Cytomegalovirus and Herpesviruses.人巨细胞病毒和疱疹病毒基因组包装后衣壳成熟与稳定过程中的结构及不同机制
Life (Basel). 2021 Feb 16;11(2):150. doi: 10.3390/life11020150.
9
Overview of Human Cytomegalovirus Pathogenesis.人类巨细胞病毒发病机制概述。
Methods Mol Biol. 2021;2244:1-18. doi: 10.1007/978-1-0716-1111-1_1.
10
Functional Profile of Human Cytomegalovirus Genes and Their Associated Diseases: A Review.人巨细胞病毒基因功能概况及其相关疾病：综述
Front Microbiol. 2020 Sep 4;11:2104. doi: 10.3389/fmicb.2020.02104. eCollection 2020.