CIST-Centro de Investigación en Salud para el Trópico, Facultad de Medicina, Universidad Cooperativa de Colombia, Santa Marta 470003, Colombia.
Grupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de Salud, Bogota 111321, Colombia.
Viruses. 2023 Apr 13;15(4):953. doi: 10.3390/v15040953.
The family comprises positive-sense RNA viruses, which mainly infect plants. Recently, a few -like viruses have been found in mosquitoes, which feed on vertebrate sources. We describe a novel -like virus, putatively named, (GUAV), isolated from and species of mosquitoes and collected in the rural area of Santa Marta, Colombia. After a cytopathic effect was observed in C6/36 cells, RNA was extracted and processed through the NetoVIR next-generation sequencing protocol, and data were analyzed through the VirMAP pipeline. Molecular and phenotypic characterization of the GUAV was achieved using a 5'/3' RACE, transmission electron microscopy, amplification in vertebrate cells, and phylogenetic analysis. A cytopathic effect was observed in C6/36 cells three days post-infection. The GUAV genome was successfully assembled, and its polyadenylated 3' end was corroborated. GUAV shared only 54.9% amino acid identity with its closest relative, , and was grouped with the latter and other unclassified insect-associated tymoviruses in a phylogenetic analysis. GUAV is a new member of a family previously described as comprising plant-infecting viruses, which seem to infect and replicate in mosquitoes. The sugar- and blood-feeding behavior of the spp., implies a sustained contact with plants and vertebrates and justifies further studies to unravel the ecological scenario for transmission.
该家族包括正链 RNA 病毒，主要感染植物。最近，在以脊椎动物为食的蚊子中发现了几种类似的病毒。我们描述了一种新型类似病毒，暂定名为，（GUAV），从哥伦比亚圣玛尔塔农村地区采集的 和 蚊子中分离出来。在 C6/36 细胞中观察到细胞病变效应后，提取 RNA，并通过 NetoVIR 下一代测序方案进行处理，通过 VirMAP 管道进行数据分析。通过 5'/3' RACE、透射电子显微镜、在脊椎动物细胞中的扩增以及系统发育分析对 GUAV 的分子和表型特征进行了研究。感染后三天，C6/36 细胞中观察到细胞病变效应。成功组装了 GUAV 基因组，并证实了其多聚腺苷酸化 3'末端。GUAV 与最接近的亲缘病毒，仅共享 54.9%的氨基酸同一性，与后者和其他未分类的昆虫相关 tymoviruses 在系统发育分析中分组。GUAV 是一个以前被描述为包含感染植物的病毒的家族的新成员，这些病毒似乎在蚊子中感染和复制。 spp. 的糖和血食行为意味着与植物和脊椎动物的持续接触，这证明有必要进一步研究以揭示传播的生态情景。
