Acta Virol. 2022;66(3):254-262. doi: 10.4149/av_2022_310.
Purple passion fruit is one of the most important fruit exports of Colombia, but its productivity is being compromised by the emergence of several viral diseases. High-throughput sequencing (HTS) surveys of viruses in purple passion fruit fields in the province of Antioquia suggested infection by a new member of the family Tymoviridae. In this work, we characterize the complete genome sequence of this virus, tentatively named purple passionfruit leaf deformation virus (PpLDV), and evaluate its distribution in Antioquia. PpLDV was assembled at high coverage in four datasets from different regions. The 6.1 kb genome of PpLDV encodes a single polyprotein with domains characteristic of the family Tymoviridae, contains a marafibox-like promoter and the 3'-UTR can fold into a tRNA-like secondary structure with a valine anti-codon. Phylogenetic analysis of the polyprotein revealed that PpLDV is a distinct member of the family Tymoviridae, more closely related to the genus Tymovirus and the unclassified Poinsettia mosaic virus (PnMV). The presence of PpLDV was confirmed by RT-qPCR and RT-PCR in samples from commercial purple passion fruit fields, plantlets and seed sprouts collected in Antioquia using primers designed in this study. Keywords: high-throughput sequencing; Marafivirus; Passifloraceae; plant virology; RT-qPCR; Tymovirus.
紫果西番莲是哥伦比亚最重要的水果出口品种之一，但由于几种病毒病的出现，其生产力受到了影响。安蒂奥基亚省紫果西番莲田病毒的高通量测序（HTS）调查表明，一种新型 Tymoviridae 家族成员感染了该病毒。在这项工作中，我们对该病毒的全基因组序列进行了特征描述，暂定名为紫西番莲叶变形病毒（PpLDV），并评估了其在安蒂奥基亚的分布情况。在四个来自不同地区的数据集上，PpLDV 以高覆盖率组装。PpLDV 的 6.1 kb 基因组编码一个单一的多聚蛋白，具有 Tymoviridae 家族的特征结构域，包含一个类似于 marafibox 的启动子，3'-UTR 可以折叠成具有缬氨酸反密码子的 tRNA 样二级结构。多聚蛋白的系统发育分析表明，PpLDV 是 Tymoviridae 家族的一个独特成员，与 Tymovirus 属和未分类的 Poinsettia mosaic virus（PnMV）更为密切相关。使用本研究设计的引物，通过 RT-qPCR 和 RT-PCR 在安蒂奥基亚收集的商业紫果西番莲田、种苗和种子芽样品中证实了 PpLDV 的存在。关键词：高通量测序；Marafivirus；西番莲科；植物病毒学；RT-qPCR；Tymovirus。
