一种用于革兰氏阳性菌高通量筛选的快速DNA提取方法。
A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria.
作者信息
Chen Nuo, Yuan Xiaoming
机构信息
Department of Food Science & Technology, Institute of Food Safety and Nutrition, Jinan University, Guangzhou, 510632, China.
Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, China.
出版信息
Bio Protoc. 2023 Apr 20;13(8):e4653. doi: 10.21769/BioProtoc.4653.
In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the screening of mutants generated in random mutagenesis. This method is effective in genomic DNA extraction for PCR amplification in several Gram-positive bacteria, including , and .
在本研究中，开发了一种基于超声处理的DNA提取方法，整个过程可在10分钟内完成。该方法几乎零成本且节省时间，适用于高通量筛选，特别是在随机诱变产生的突变体筛选中。该方法在几种革兰氏阳性细菌（包括 、 和 ）的基因组DNA提取中对PCR扩增有效。
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