印度尼西亚结直肠癌患者 NRAS 突变和 HER2 过表达的不同临床病理特征。
Different Clinicopathological Characteristics in Indonesian Colorectal Patients with NRAS Mutations and HER2 Over-Expression.
机构信息
Department of Surgery, Faculty of Medicine, Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Basic Medical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
出版信息
Asian Pac J Cancer Prev. 2023 Apr 1;24(4):1373-1377. doi: 10.31557/APJCP.2023.24.4.1373.
OBJECTIVE
This study aims to assess the association of subject characteristics and NRAS mutations with HER2 expression in CRC.
METHODS
This research is a cross-sectional study. The research subjects in this study were colorectal cancer patients in the Digestive Surgery division at Dr. Hasan Sadikin Hospital. There were 58 study subjects. Examination of NRAS mutations was carried out by Polymerase Chain Reaction (PCR) from fresh tumour tissue obtained from surgery or colonoscopy. Meanwhile, HER2 examination used the Immunohistochemistry (IHC) method of paraffin blocks for anatomical pathology examination of the same patients.
RESULT
HER2 overexpression was found in 6/58 (10.3%) patients with CRC, and from 8 subjects with NRAS mutations, only 1 subject (1.7%) showed overexpression of HER2. Univariate analysis of HER2 expression showed no significant associations to age, sex, histologic feature, tumor location, and NRAS mutations. A significant association was found between HER2 expression and stage of the CRC with p=0.001.
CONCLUSION
There is no association between NRAS mutations and HER2 overexpression in colorectal cancer patients.
目的
本研究旨在评估患者特征和 NRAS 突变与 CRC 中 HER2 表达的相关性。
方法
这是一项横断面研究。本研究的研究对象为 Hasan Sadikin 医院消化外科的结直肠癌患者。共纳入 58 例患者。通过从手术或结肠镜获得的新鲜肿瘤组织进行聚合酶链反应（PCR）检测 NRAS 突变。同时，使用免疫组织化学（IHC）方法对石蜡块进行 HER2 检测，这些石蜡块来自同一批接受解剖病理学检查的患者。
结果
CRC 患者中有 6/58（10.3%）例出现 HER2 过表达，而在 8 例存在 NRAS 突变的患者中，仅有 1 例（1.7%）出现 HER2 过表达。HER2 表达的单因素分析显示与年龄、性别、组织学特征、肿瘤位置和 NRAS 突变无关。HER2 表达与 CRC 分期显著相关（p=0.001）。
结论
结直肠癌患者中 NRAS 突变与 HER2 过表达之间无相关性。
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