Jazrawi S P, Horton R W
J Neurochem. 1986 Jul;47(1):173-7. doi: 10.1111/j.1471-4159.1986.tb02846.x.
We have examined if the age-related susceptibility of DBA/2J mice to audiogenic seizures is the result of an abnormality in the number or sensitivity of brain adrenoceptors. The binding of alpha 1-, alpha 2-, and beta-adrenoceptor ligands to membranes prepared from whole brain or regions of brain of DBA/2J mice was measured at various ages, corresponding to the periods before, during, and after the maximal sensitivity to audiogenic seizures. For comparison, we have studied concurrently age-matched C57 Bl/6 mice, a strain resistant to audiogenic seizures at all ages. There was no difference in the binding of alpha 2- or beta-adrenoceptor ligands to whole brain membranes between the two strains of mice at any age. The maximal number of alpha 1-adrenoceptor binding sites was lower in whole brains of DBA/2J mice than of C57 Bl/6 mice at all ages studied except 13-15 days of age. The differences were small (maximally 17%) but were statistically significant at 21-23 days of age, the time of maximal sensitivity of DBA/2J mice to audiogenic seizures. No difference between the two strains was found in the number or affinity of alpha 1- or alpha 2-adrenoceptor binding sites at any age in any of the brain regions studied. The age-related susceptibility of DBA/2J mice to audiogenic seizures is not the result of an abnormality in number or sensitivity of alpha 2- or beta-adrenoceptor binding sites, but a reduced number of alpha 1-adrenoceptor binding sites may be involved.
我们研究了DBA/2J小鼠与年龄相关的对听源性癫痫发作的易感性是否是由于脑肾上腺素能受体数量或敏感性异常所致。在不同年龄阶段，测定了α1、α2和β肾上腺素能受体配体与DBA/2J小鼠全脑或脑区制备的膜的结合情况，这些年龄阶段分别对应于对听源性癫痫发作最大敏感性之前、期间和之后的时期。为作比较，我们同时研究了年龄匹配的C57 Bl/6小鼠，该品系在所有年龄段均对听源性癫痫发作有抗性。在任何年龄，两种品系小鼠的α2或β肾上腺素能受体配体与全脑膜的结合均无差异。在所研究的所有年龄中，除13 - 15日龄外，DBA/2J小鼠全脑中α1肾上腺素能受体结合位点的最大数量均低于C57 Bl/6小鼠。差异较小（最大为17%），但在21 - 23日龄时具有统计学意义，这是DBA/2J小鼠对听源性癫痫发作敏感性最高的时期。在所研究的任何脑区，两种品系在任何年龄的α1或α2肾上腺素能受体结合位点的数量或亲和力均未发现差异。DBA/2J小鼠与年龄相关的对听源性癫痫发作的易感性并非α2或β肾上腺素能受体结合位点数量或敏感性异常的结果，但可能涉及α1肾上腺素能受体结合位点数量的减少。
