College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124624. doi: 10.1016/j.ijbiomac.2023.124624. Epub 2023 Apr 28.
A soybean protein isolate (SPI)-apricot polysaccharide gel with hypolipidemic activity that can be used for 3D printing was prepared and the mechanism of its gel formation was studied in this work. The results demonstrated that adding apricot polysaccharide to SPI could effectively improve the bound water content, viscoelastic properties and rheological properties of the gels. Low-field NMR, FT-IR spectroscopy and surface hydrophobicity confirmed that the interactions between SPI and apricot polysaccharide were mainly realized by electrostatic interactions, hydrophobic and hydrogen bonding. Furthermore, adding modified polysaccharide treated by ultrasonic-assisted Fenton method to SPI on the basis of low-concentration apricot polysaccharide contributed to improving the 3D printing accuracy and stability of the gel. Consequently, the gel formed by adding apricot polysaccharide (0.5 %, m/v) and modified polysaccharide (0.1 %, m/v) to SPI had the best hypolipidemic activity (the binding rate of sodium taurocholate and sodium glycocholate were 75.33 % and 72.86 %, respectively) and 3D printing characteristics.
本文制备了一种具有降血脂活性的大豆分离蛋白-杏多糖凝胶,可用于 3D 打印,并研究了其凝胶形成的机制。结果表明,向 SPI 中添加杏多糖可以有效提高凝胶的结合水含量、黏弹性和流变性能。低场 NMR、傅里叶变换红外光谱和表面疏水性证实,SPI 和杏多糖之间的相互作用主要通过静电相互作用、疏水相互作用和氢键实现。此外,在低浓度杏多糖的基础上,添加经超声辅助 Fenton 法处理的改性多糖,有助于提高凝胶的 3D 打印精度和稳定性。因此,向 SPI 中添加杏多糖(0.5%,m/v)和改性多糖(0.1%,m/v)形成的凝胶具有最佳的降血脂活性(牛磺胆酸钠和甘胆酸钠的结合率分别为 75.33%和 72.86%)和 3D 打印特性。