Clinical Laboratory Medicine Department, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, PR China; MOE & Guangdong Provincial Key Laboratory of Laser Life Science, Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, PR China.
MOE & Guangdong Provincial Key Laboratory of Laser Life Science, Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, PR China.
Biosens Bioelectron. 2023 Aug 15;234:115353. doi: 10.1016/j.bios.2023.115353. Epub 2023 Apr 25.
Lateral flow assays (LFAs) are promising points-of-care tests, playing a vital role in diseases screening, diagnosis and surveillance. However, development of portable, cheap, and smart LFAs platform for sensitive and accurate quantification of disease biomarkers in complex media is challenging. Here, a cheap handheld device was developed to realize on-site detection of disease biomarkers by Nd/Yb co-doped near-infrared (NIR)-to-NIR downconversion nanoparticles (DCNPs) based LFA. Its sensitivity is at least 8-fold higher for detecting NIR light signal from Nd/Yb co-doped nanoparticles than conventional expensive InGaAs camera based detection platform. Additionally, we enhance NIR quantum yield of Nd/Yb co-doped nanoparticles up to 35.5% via simultaneous high dopant of sensitizer ions Nd and emitter ions Yb. Combination of NIR-to-NIR handheld detection device and ultra-bright NIR emitting NaNbF:Yb60%@NaLuF nanoparticle probe allows the detection sensitivity of SARS-CoV-2 ancestral strain and Omicron variants specific neutralizing antibodies LFA up to the level of commercial enzyme linked immunosorbent assay kit. Furthermore, by this robust method, enhanced neutralizing antibodies against SARS-CoV-2 ancestral strain and Omicron variants are observed in healthy participants with Ad5-nCoV booster on top of two doses of inactivated vaccine. This NIR-to-NIR handheld platform provides a promising strategy for on-site evaluating protective humoral immunity after SARS-CoV-2 vaccination or infection.
侧向流动检测（LFA）是一种很有前途的即时检测手段，在疾病筛查、诊断和监测方面发挥着重要作用。然而，开发用于在复杂介质中对疾病生物标志物进行灵敏、准确定量的便携式、廉价和智能 LFA 平台仍然具有挑战性。在这里，我们开发了一种廉价的手持式设备，通过基于 Nd/Yb 共掺杂近红外（NIR）-NIR 下转换纳米粒子（DCNP）的 LFA 实现疾病生物标志物的现场检测。与传统的昂贵的基于 InGaAs 相机的检测平台相比，该设备检测 Nd/Yb 共掺杂纳米粒子的 NIR 光信号的灵敏度至少提高了 8 倍。此外，我们通过同时高掺杂敏化剂离子 Nd 和发射体离子 Yb，将 Nd/Yb 共掺杂纳米粒子的 NIR 量子产率提高到 35.5%。NIR-to-NIR 手持式检测装置与超亮 NIR 发射 NaNbF:Yb60%@NaLuF 纳米颗粒探针的结合，使 SARS-CoV-2 原始株和奥密克戎变异株特异性中和抗体 LFA 的检测灵敏度达到商业酶联免疫吸附测定试剂盒的水平。此外，通过这种稳健的方法，在接种两剂灭活疫苗的基础上，在接受 Ad5-nCoV 加强针的健康参与者中观察到针对 SARS-CoV-2 原始株和奥密克戎变异株的增强型中和抗体。这种 NIR-to-NIR 手持式平台为评估 SARS-CoV-2 疫苗接种或感染后的保护性体液免疫提供了一种有前景的策略。
