在大鼠和兔子皮肤涂抹两种14C标记的偶氮染料后的放射性排泄情况。

Excretion of radioactivity from rats and rabbits following cutaneous application of two 14C-labeled azo dyes.

作者信息

Aldrich F D, Busby W F, Fox J G

出版信息

J Toxicol Environ Health. 1986;18(3):347-55. doi: 10.1080/15287398609530876.

Abstract

A benzidine-derived azo dye, C.I. direct black 38 (DB38), and a p-phenylenediamine-derived dye, C.I. direct black 19 (DB19), labeled with carbon-14 in their aromatic amine moieties, were applied to the shaved dorsal skin of male Fischer-344 rats and New Zealand rabbits. Application sites were protected with nylon gauze and elastic bandage assemblies. Following application of measured amounts of radiolabeled dye in 0.1 M pH 10.2 carbonate buffer, serial urine and fecal samples were obtained from individual animals in metabolism cages at 24, 48, 72, 96, 120, and 144 h. Aliquots of urine and fecal homogenates were assayed for radioactivity by scintillation counting. Cumulative excretion of radioactivity from rats receiving DB38 was 0.05% of total dermal dose at 144 h in urine, and 0.16% of total dermal dose in feces. Cumulative excretion of radioactivity from DB38-treated rabbits at 144 h was 3.12% of total dermal dose in urine, and 5.12% in feces. From rats and rabbits receiving topical DB19, cumulative excretion of radioactivity at 144 h was less than that from DB38-treated animals. In rat urine, 0.04% of total dermal dose appeared; in rat feces, no radioactivity was recovered. In rabbit urine, 0.04% of dermal dose was found; 0.01% appeared in rabbit feces.

摘要

一种联苯胺衍生的偶氮染料，即 C.I. 直接黑 38（DB38），以及一种对苯二胺衍生的染料，即 C.I. 直接黑 19（DB19），其芳香胺部分用碳 - 14 标记，被涂抹于雄性 Fischer - 344 大鼠和新西兰兔剃毛的背部皮肤。涂抹部位用尼龙纱布和弹性绷带组件进行保护。在 0.1 M pH 10.2 的碳酸盐缓冲液中加入测量量的放射性标记染料进行涂抹后，在 24、48、72、96、120 和 144 小时从代谢笼中的个体动物获取连续的尿液和粪便样本。尿液和粪便匀浆的等分试样通过闪烁计数法测定放射性。接受 DB38 的大鼠在 144 小时时，尿液中放射性的累积排泄量为皮肤总剂量的 0.05%，粪便中为 0.16%。接受 DB38 处理的兔子在 144 小时时，尿液中放射性的累积排泄量为皮肤总剂量的 3.12%，粪便中为 5.12%。接受局部 DB19 的大鼠和兔子在 144 小时时放射性的累积排泄量低于接受 DB38 处理的动物。在大鼠尿液中，出现了皮肤总剂量的 0.04%；在大鼠粪便中，未检测到放射性。在兔子尿液中，发现了皮肤剂量的 0.04%；在兔子粪便中出现了 0.01%。