Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, OR 97213, USA.
Thermo Fisher Scientific, Waltham, MA 02451, USA.
Bioanalysis. 2023 Apr;15(8):429-447. doi: 10.4155/bio-2023-0005. Epub 2023 May 1.
Previously designed flow cytometry panels have provided a framework to analyze T-cell activation; however, few provide an extensive view of lymphocyte populations, and none are optimized for murine models. This article describes a panel designed specifically to assess the expression of activation and exhaustion markers in expanding lymphocyte populations in tumor-bearing mice across two distinct genetic backgrounds: BALB/c and C57BL/6. This comprehensive panel enables the assessment of multiple functional states and immune checkpoint markers across cytotoxic CD8 T cells, helper and regulatory CD4 T cells and natural killer cells in murine whole blood, lymph nodes and tumor.
先前设计的流式细胞术面板为分析 T 细胞激活提供了一个框架；然而，很少有面板能广泛地观察淋巴细胞群体，也没有专门针对小鼠模型进行优化的面板。本文描述了一个专门设计的面板，用于评估荷瘤小鼠在两种不同遗传背景（BALB/c 和 C57BL/6）下扩增的淋巴细胞群体中激活和耗竭标志物的表达。这个全面的面板能够评估小鼠全血、淋巴结和肿瘤中细胞毒性 CD8 T 细胞、辅助和调节性 CD4 T 细胞以及自然杀伤细胞的多种功能状态和免疫检查点标志物。
