Faculty of Medicine, Department of Transfusiology, University in Novi Sad, Novi Sad, Serbia.
Department of Laboratory Diagnostics, Blood Transfusion Institute of Vojvodina, Novi Sad, Serbia.
Vox Sang. 2023 Jun;118(6):497-501. doi: 10.1111/vox.13431. Epub 2023 May 2.
In the era of gel cards, we aimed to determine the incidence of a positive direct antiglobulin test (DAT) in a population of blood donors in Serbia and to compare results with the period when a conventional tube technique was used.
A retrospective study was conducted on 184,171 blood donors over 10 years (2012-2021). Positive DAT results initially were detected after positive result of the compatibility test. After a DAT had been initially performed on fresh blood samples, a control DAT was performed 6 months later.
Using gel cards, we found a low incidence (0.09%) of DAT positivity among 55,254 female and 128,917 male blood donors, with no difference found in the occurrence of a positive DAT between them. Positive DAT moderately positively correlated with increasing age of blood donors, but without statistical significance. The vast majority of blood donors (98.86%) were IgG positive. All donors were in good health, did not take medication or had anaemia or haemolysis. During the period 1980-1989, when the test tube technique was used, we found 0.05% DAT-positive blood donors.
The incidence of positive DAT results in Serbian blood donors is higher compared with that found using the previous test tube technique, but the incidence is consistent with the findings of other studies.
在胶卡时代，我们旨在确定塞尔维亚献血人群中直接抗球蛋白试验（DAT）阳性的发生率，并将结果与使用传统试管技术的时期进行比较。
对 10 年间（2012 年至 2021 年）的 184171 名献血者进行了回顾性研究。最初在相容性试验阳性后检测到 DAT 阳性结果。在对新鲜血样进行初始 DAT 检测后，6 个月后进行了对照 DAT 检测。
使用胶卡，我们发现 55254 名女性和 128917 名男性献血者中 DAT 阳性率较低（0.09%），两者之间未发现 DAT 阳性的发生存在差异。阳性 DAT 与献血者年龄的增加呈中度正相关，但无统计学意义。绝大多数献血者（98.86%）为 IgG 阳性。所有献血者身体健康，未服用药物，无贫血或溶血。在 1980-1989 年使用试管技术的时期，我们发现 0.05%的 DAT 阳性献血者。
与使用之前的试管技术相比，塞尔维亚献血者阳性 DAT 结果的发生率较高，但与其他研究的结果一致。
