Department of Food Science and Nutrition, California Polytechnic State University, San Luis Obispo, California 93407, United States.
College of Agriculture, Food and Environmental Sciences, California Polytechnic State University, San Luis Obispo, California 93407, United States.
J Proteome Res. 2023 Jun 2;22(6):1603-1613. doi: 10.1021/acs.jproteome.2c00430. Epub 2023 May 2.
Gestational Diabetes Mellitus (GDM) results in complications affecting both mothers and their offspring. Metabolomic analysis across pregnancy provides an opportunity to better understand GDM pathophysiology. The objective was to conduct a metabolomics analysis of first and third trimester plasma samples to identify metabolic differences associated with GDM development. Forty pregnant women with overweight/obesity from a multisite clinical trial of a lifestyle intervention were included. Participants who developed GDM ( = 20; GDM group) were matched with those who did not develop GDM ( = 20; Non-GDM group). Plasma samples collected at the first (10-16 weeks) and third (28-35 weeks) trimesters were analyzed with ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Cardiometabolic risk markers, dietary recalls, and physical activity metrics were also assessed. Four medium-chain acylcarnitines, lauroyl-, octanoyl-, decanoyl-, and decenoylcarnitine, significantly differed over the course of pregnancy in the GDM vs Non-GDM group in a group-by-time interaction ( < 0.05). Hypoxanthine and inosine monophosphate were elevated in the GDM group ( < 0.04). In both groups over time, bile acids and sorbitol increased while numerous acylcarnitines and α-hydroxybutyrate decreased ( < 0.05). Metabolites involved in fatty acid oxidation and purine degradation were altered across the first and third trimesters of GDM-affected pregnancies, providing insight into metabolites and metabolic pathways altered with GDM development.
妊娠期糖尿病（GDM）可导致母婴双方并发症。对妊娠期间的代谢组学分析提供了更好地理解 GDM 病理生理学的机会。本研究的目的是对第一和第三孕期的血浆样本进行代谢组学分析，以确定与 GDM 发展相关的代谢差异。研究纳入了来自多中心生活方式干预临床试验的超重/肥胖孕妇 40 例。发生 GDM 的参与者（n = 20；GDM 组）与未发生 GDM 的参与者（n = 20；非 GDM 组）相匹配。在第一（10-16 周）和第三（28-35 周）孕期收集的血浆样本采用超高效液相色谱-质谱法（UPLC-MS）进行分析。还评估了心血管代谢风险标志物、膳食回顾和身体活动指标。在 GDM 组与非 GDM 组中，4 种中链酰基辅酶 A（lauroyl-、octanoyl-、decanoyl- 和 decenoylcarnitine）在妊娠过程中随时间的变化存在组间时间交互作用（ < 0.05）。在 GDM 组中，次黄嘌呤和肌苷单磷酸升高（ < 0.04）。在两组中，随时间推移，胆汁酸和山梨醇增加，而许多酰基辅酶 A 和 α-羟丁酸减少（ < 0.05）。妊娠第一和第三孕期中，与脂肪酸氧化和嘌呤降解相关的代谢物发生改变，这为了解 GDM 发展过程中代谢物和代谢途径的改变提供了依据。
