Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Biomol NMR Assign. 2023 Jun;17(1):107-114. doi: 10.1007/s12104-023-10128-4. Epub 2023 May 2.
Rev7 is a versatile HORMA (Hop1, Rev7, Mad2) family adaptor protein with multiple roles in mitotic regulation and DNA damage response, and an essential accessory subunit of the translesion synthesis (TLS) DNA polymerase Polζ employed in replication of damaged DNA. Within Polζ, the two copies of Rev7 interact with the two Rev7-bonding motifs (RBM1 and RBM2) of the catalytic subunit Rev3 by a mechanism characteristic of HORMA proteins whereby the "safety-belt" loop of Rev7 closes on the top of the ligand. Here we report the nearly complete backbone and Ile, Val, Leu side-chain methyl NMR resonance assignments of the 27 kDa human Rev7/Rev3-RBM1 and Rev7/Rev3-RBM2 complexes (BMRB deposition numbers 51651 and 51652) that will facilitate future NMR studies of Rev7 dynamics and interactions.
Rev7 是一种多功能的 HORMA（Hop1、Rev7、Mad2）家族衔接蛋白，在有丝分裂调控和 DNA 损伤反应中具有多种作用，并且是在受损 DNA 复制中使用的跨损伤合成（TLS）DNA 聚合酶 Polζ 的必需辅助亚基。在 Polζ 中，Rev7 的两个拷贝通过 HORMA 蛋白特有的机制与催化亚基 Rev3 的两个 Rev7 结合基序（RBM1 和 RBM2）相互作用，Rev7 的“安全带”环封闭在配体的顶部。在这里，我们报告了 27 kDa 人 Rev7/Rev3-RBM1 和 Rev7/Rev3-RBM2 复合物（BMRB 沉积编号 51651 和 51652）的几乎完整的骨架和 Ile、Val、Leu 侧链甲基 NMR 共振分配，这将有助于未来 Rev7 动力学和相互作用的 NMR 研究。
