Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Appl Biochem Biotechnol. 2024 May;196(5):2591-2611. doi: 10.1007/s12010-023-04502-0. Epub 2023 May 2.
Production of value-added products from waste anaerobic digestate is economically and environmentally important for sustainable development of industrial process and products. In this study halophilic microalgae, Chlorella vulgaris 92001, Chlorella vulgaris 50291, Chlorella vulgaris 10241 and Tetraselmis indica, were initially screened for lutein production using synthetic dairy digestate (DD), municipal digestate (MD) and poultry digestate (PD) as no-cost substrates. Screening and optimization of parameters, such as dilution, pH, MgCl, NaCl, NaHCO and inoculum concentration for maximum lutein production were further performed employing statistically designed Plackett-Burman and response surface methodology. Cultivation of C. vulgaris 92001 in a split column photobioreactor under optimum culture condition showed increase in lutein production by 2.36-fold in batch mode. The influence of different hydraulic retention time (HRT) values of 150, 130, 100 and 90 h on lutein production was evaluated in continuous mode with the split column photobioreactor. Lutein produced using the synthetic poultry digestate showed good potential biopesticide activity against Spodoptera litura (fall armyworm). Overall, this study demonstrated bioprocess development to produce lutein using synthetic anaerobic digestate from marine algae and its potential application as a biopesticide.
利用废厌氧消化物生产高附加值产品，对于工业过程和产品的可持续发展具有重要的经济和环境意义。本研究初步筛选了嗜盐微藻小球藻 92001、小球藻 50291、小球藻 10241 和盐生杜氏藻，以合成乳制品消化物（DD）、市政消化物（MD）和家禽消化物（PD）作为无成本底物生产叶黄素。采用统计学设计的 Plackett-Burman 和响应面法进一步筛选和优化参数，如稀释度、pH 值、MgCl2、NaCl、NaHCO3 和接种物浓度，以获得最大叶黄素产量。在最佳培养条件下，将小球藻 92001 培养在分裂柱光生物反应器中，在分批培养模式下，叶黄素产量提高了 2.36 倍。在连续模式下，用分裂柱光生物反应器评估了不同水力停留时间（HRT）值 150、130、100 和 90 h 对叶黄素生产的影响。使用合成家禽消化物生产的叶黄素对斜纹夜蛾（草地贪夜蛾）表现出良好的潜在生物农药活性。总的来说，这项研究展示了利用海洋藻类的合成厌氧消化物生产叶黄素的生物工艺开发及其作为生物农药的潜在应用。
