壳寡糖-儿茶素缀合物负载脂质体使用不同稳定剂的特性、稳定性和生物活性。
Chitooligosaccharide-catechin conjugate loaded liposome using different stabilising agents: characteristics, stability, and bioactivities.
机构信息
International Center of Excellence in Seafood Science and Innovation (ICE-SSI), Faculty of Agro-Industry, Prince of Songkla University, Songkhla, Hat Yai, Thailand.
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
出版信息
J Microencapsul. 2023 Sep;40(6):385-401. doi: 10.1080/02652048.2023.2209658. Epub 2023 May 9.
AIM
To determine the optimum condition for preparing chitooligosaccharide-catechin conjugate (COS-CAT) liposomes using different stabilising agents.
METHODS
COS-CAT liposomes (0.1-1%, w/v) were prepared using soy phosphatidylcholine (SPC) (50-200 mM) and glycerol or cholesterol (25-100 mg). Encapsulation efficiency (EE), loading capacity (LC), physicochemical characteristics, FTIR spectra, thermal stability, and structure of COS-CAT liposomes were assessed.
RESULTS
COS-CAT loaded liposome stabilised by cholesterol (COS-CAT-CHO) showed higher stability as shown by the highest EE (76.81%) and LC (4.57%) and the lowest zeta potential (ZP) (-76.51 mV), polydispersity index (PDI) (0.2674) and releasing efficiency (RE) (53.54%) ( < 0.05). COS-CAT-CHO showed the highest retention and relative remaining bioactivities of COS-CAT under various conditions ( < 0.05). FTIR spectra revealed the interaction between the choline group of SPC and -OH groups of COS-CAT. Phase transition temperature of COS-CAT-CHO was shifted to 184 °C, which was higher than others ( < 0.05).
CONCLUSION
SPC and cholesterol-based liposome could be used as a promising vesicle for maintaining bioactivities of COS-CAT.
目的
确定使用不同稳定剂制备壳寡糖-儿茶素缀合物（COS-CAT）脂质体的最佳条件。
方法
使用大豆卵磷脂（SPC）（50-200mM）和甘油或胆固醇（25-100mg）制备 0.1-1%（w/v）的 COS-CAT 脂质体（0.1-1%，w/v）。评估包封效率（EE）、载药量（LC）、物理化学特性、傅里叶变换红外光谱（FTIR）、热稳定性和 COS-CAT 脂质体的结构。
结果
用胆固醇稳定的负载 COS-CAT 的脂质体（COS-CAT-CHO）显示出更高的稳定性，表现为最高的 EE（76.81%）和 LC（4.57%）以及最低的 ZP（-76.51mV）、PDI（0.2674）和 RE（53.54%）（ < 0.05）。COS-CAT-CHO 表现出在各种条件下（ < 0.05）COS-CAT 保留率和相对剩余生物活性最高。FTIR 光谱显示了 SPC 的胆碱基团与 COS-CAT 的-OH 基团之间的相互作用。COS-CAT-CHO 的相变温度升高至 184°C，高于其他脂质体（ < 0.05）。
结论
基于 SPC 和胆固醇的脂质体可作为维持 COS-CAT 生物活性的有前途的囊泡。
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