谷胱甘肽 S-转移酶 M1 基因多态性与胃癌的关系。

Department of Digestive Medicine, Jinan City Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, China.

Emergency Infusion Room, Jinan City Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, China.

Cell Mol Biol (Noisy-le-grand). 2022 Dec 31;68(12):49-53. doi: 10.14715/cmb/2022.68.12.10.

This experiment was designed to investigate the relationship between glutathione S-transferase M1 (GSTM1) gene polymorphism and gastric cancer (GC). For this purpose, from January 2013 to December 2014, 116 patients with GC diagnosed in the Department of Gastroenterology of our hospital and 120 healthy people in the physical examination center were selected as the research objects. 116 patients with GC served as the observation group and 120 healthy people in the physical examination center served as the control group. Collect and isolate the peripheral blood nucleated cells of the subjects, obtain the GSTM1 gene polymorphism by sequencing, analyze the differences of GSTM1 genotype between the two groups, compare the differences of clinicopathological characteristics of patients with different genotypes in the observation group, look for the survival relative risk factors of patients with GC, and analyze the risk factors of death risk of GC by multivariate Cox risk proportional regression. Results showed that the proportion of GSTM1 (-) in the observation group (62.07%) was raised compared with the control group (48.33%) (p<0.05). There was a correlation between GSTM1 gene polymorphism and smoking, TNM stage differentiation and GSTM1 gene polymorphism in the observation group. The specific analysis found that the proportion of non-smoking, stage I-III and low differentiation in the GSTM1 (-) group was raised compared with that in the GSTM1 (+) group (p<0.05). TNM stage, differentiation degree and GSTM1 gene polymorphism were correlated with the median survival time of patients with GC (p<0.05). Further multivariate Cox risk proportional regression analysis showed that TNM stage IV, low differentiation and GSTM1 (-) the relative risk coefficients of death in patients with GC were stage Ⅰ - Ⅲ, high/medium differentiation and GSTM1, respectively (+) patients were 1.75, 1.46, and 2.14 times higher. In conclusion, GSTM1 gene polymorphism is associated with susceptibility to GC, and the GSTM1 deletion genotype is an unfavourable factor for poor prognosis in patients with GC.

本实验旨在探讨谷胱甘肽 S-转移酶 M1（GSTM1）基因多态性与胃癌（GC）的关系。为此，我们选取了 2013 年 1 月至 2014 年 12 月期间在我院消化内科就诊的 116 例 GC 患者和体检中心的 120 名健康人作为研究对象。将 116 例 GC 患者作为观察组，将体检中心的 120 名健康人作为对照组。收集并分离受试者外周血核细胞，通过测序获得 GSTM1 基因多态性，分析两组 GSTM1 基因型的差异，比较观察组不同基因型患者的临床病理特征差异，寻找 GC 患者的生存相关危险因素，并通过多因素 Cox 风险比例回归分析 GC 死亡风险的危险因素。结果显示，观察组 GSTM1（-）的比例（62.07%）高于对照组（48.33%）（p<0.05）。观察组 GSTM1 基因多态性与吸烟、TNM 分期分化有关。具体分析发现，GSTM1（-）组不吸烟、Ⅰ-Ⅲ期和低分化的比例高于 GSTM1（+）组（p<0.05）。TNM 分期、分化程度和 GSTM1 基因多态性与 GC 患者的中位生存时间相关（p<0.05）。进一步的多因素 Cox 风险比例回归分析显示，TNM 分期Ⅳ期、低分化和 GSTM1（-）患者的死亡相对危险系数分别为Ⅰ-Ⅲ期、高/中分化和 GSTM1（+）患者的 1.75、1.46 和 2.14 倍。综上所述，GSTM1 基因多态性与 GC 的易感性有关，GSTM1 缺失基因型是 GC 患者预后不良的不利因素。