Mode of Action of Novel Pyrazoloquinazoline on Diamondback Moth () Ligand-Gated Chloride Channels.

In our previous study, a series of novel pyrazoloquinazolines were synthesized. Pyrazoloquinazoline showed high insecticidal activity against the diamondback moth () and no cross-resistance to fipronil. Patch clamp electrophysiology performed on pupae brains and two-electrode voltage clamp electrophysiology performed on oocytes indicated that might act on the ionotropic γ-aminobutyric acid (GABA) receptor (GABAR) and glutamate-gated chloride channel (GluCl). Moreover, 's potency on GluCl was about 15-fold higher than on fipronil, which may explain why there was no cross-resistance between and fipronil. Downregulation of the transcription level significantly enhanced the insecticidal activity of on . These findings shed light on the mode of action of and provide important insights into the development of new insecticides for agricultural applications.