Cruz M A, Rudolph M I
Life Sci. 1986 Jun 2;38(22):2043-51. doi: 10.1016/0024-3205(86)90153-0.
Correlation between contractile activity and norepinephrine (NE) release induced by electrical stimulation or by high K+ depolarization has been analyzed in isolated preparations of mouse uterus throughout the different stages of the estrous cycle. Both the contractile activity induced by electrical stimulation and the capacity to maintain contracture, after changing the physiological bathing solution by high K+ solution, followed the same pattern: estrous greater than proestrous greater than diestrous greater than metestrous. High-K+ induced 3H-NE release was also different according to the stage of the estrous cycle. 3H-NE release was significantly less in estrous than in diestrous uterine horns. EC50 values for inhibiting contractile response, for isoprenaline, norepinephrine and phenylephrine were significantly greater in metestrous than in other stages of the estrous cycle. On the other hand, reserpinized mouse uteri showed an increase in EC50 values in the stages tested. The data support the hypothesis that in a mouse uterus, sex steroid hormones could affect beta-adrenergic receptor function indirectly, perhaps through an action on adrenergic neurons by a mechanism affecting NE release from sympathetic terminals.
在小鼠子宫的离体标本中，研究了发情周期不同阶段电刺激或高钾去极化诱导的收缩活动与去甲肾上腺素（NE）释放之间的相关性。电刺激诱导的收缩活动以及用高钾溶液更换生理浴液后维持挛缩的能力，均呈现相同模式：发情期大于动情前期大于动情间期大于动情后期。高钾诱导的3H-NE释放也因发情周期阶段而异。发情期子宫角的3H-NE释放显著少于动情间期。抑制异丙肾上腺素、去甲肾上腺素和苯肾上腺素收缩反应的EC50值在动情后期显著高于发情周期的其他阶段。另一方面，利血平化的小鼠子宫在测试阶段的EC50值升高。这些数据支持这样的假设，即在小鼠子宫中，性甾体激素可能间接影响β-肾上腺素能受体功能，可能是通过影响交感神经末梢NE释放的机制作用于肾上腺素能神经元。
