Montesino H, Villar M, Vega E, Rudolph M I
Departamento de Farmacología, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
Biochem Pharmacol. 1995 Jul 31;50(3):407-11. doi: 10.1016/0006-2952(95)00140-u.
The effect of histamine on [3H]norepinephrine ([3H]NE) release in uterine horns from mice in estrous and diestrous states was studied under two different experimental conditions: resting NE release and stimulus-evoked NE release. It was found that [3H]NE release was higher for the diestrous state under both resting and stimulus-evoked (100 mM K+ and electrical stimulus) conditions. Histamine only potentiated the stimulus-evoked [3H]NE outflow in uterine horns from mice in the diestrous state and from ovariectomized mice treated with progesterone. This effect was dose dependent and was antagonized by H1 but not by H2 or H3 antagonists. R-alpha-Methylhistamine, a H3 agonist, has no effect on stimulus-evoked [3H]NE release. According to these results, it could be concluded that: (a) histamine regulates the NE release from noradrenergic nerve terminals in uterine tissues; (b) this heterologous regulation depends on progesterone predominance and on terminal depolarization; and (c) presynaptic H1 receptors located on noradrenergic terminals could be responsible for such an effect.
在两种不同实验条件下，即静息去甲肾上腺素（NE）释放和刺激诱发的NE释放，研究了组胺对处于动情期和动情间期的小鼠子宫角中[³H]去甲肾上腺素（[³H]NE）释放的影响。发现在静息和刺激诱发（100 mM钾离子和电刺激）条件下，动情间期的[³H]NE释放均较高。组胺仅增强了处于动情间期的小鼠以及用孕酮处理的去卵巢小鼠子宫角中刺激诱发的[³H]NE流出。这种作用呈剂量依赖性，且被H1拮抗剂拮抗，而不被H2或H3拮抗剂拮抗。H3激动剂R-α-甲基组胺对刺激诱发的[³H]NE释放无影响。根据这些结果，可以得出以下结论：（a）组胺调节子宫组织中去甲肾上腺素能神经末梢的NE释放；（b）这种异源调节取决于孕酮优势和终末去极化；（c）位于去甲肾上腺素能终末的突触前H1受体可能是造成这种作用的原因。
