Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL-CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, P.O. Box 20, El Jadida, M-24000, Morocco.
BMC Plant Biol. 2023 May 3;23(1):234. doi: 10.1186/s12870-023-04249-0.
In this study, the ecological conditions of the natural habitat of Lemna minuta Kunth in Morocco were investigated, and the impact of five synthetic growth media (Murashige-Skoog (MS), Schenk-Hildebrand (SH), Hoagland medium (HM), 10X Algal Assay Procedure (AAP), and Swedish Standard Institute medium (SIS)) on the morphophysiological and biochemical parameters was analysed. The morphophysiological parameters included root length, frond surface area, and fresh weight, while the biochemical parameters included photosynthetic pigments, carbohydrates, and protein content. The study was conducted in vitro in two phases: an uncontrolled aeration system (Phase I) and a controlled aeration system (Phase II).The results showed that the pH, conductivity, salinity, and ammonium levels in the natural habitat were within the optimal range for duckweed growth. The measured orthophosphate concentrations were higher compared to previous observations, while the recorded chemical oxygen demand values were low. The study also revealed a significant effect of the culture medium composition on the morphophysiological and biochemical parameters of the duckweed. The fresh weight biomass, relative growth rate in fronds, relative growth rate in surface area, root length, protein content, carbohydrates, chlorophyll (a), chlorophyll (b), total chlorophyll, carotenoids, and the chlorophyll (a/b) ratio were all affected by the culture medium.The most accurate regression models described the growth index GI(F) based on time and in vitro culture conditions in both phases. In Phase I, the best models for MS, SIS, AAP, and SH media were linear, weighted quadratic, cubic, and weighted cubic, respectively. In Phase II, the best models for all growth media were linear. The time coefficients (in days) for Phase II were 0.321, 0.547, 1.232, 1.470, and 0.306 for AAP, HM, MS, SH, and SIS, respectively.Comparing the morphophysiological and biochemical parameters of fronds from different media and analysing the regression model results showed that the SH and MS media were the best among the tested media for the in vitro culture of L. minuta in controlled aeration conditions. However, further research is needed to develop new synthetic media that best promote the growth and maintenance of this duckweed in long-term culture.
在这项研究中，我们调查了摩洛哥产的浮萍自然栖息地的生态条件，并分析了五种合成生长培养基（Murashige-Skoog (MS)、Schenk-Hildebrand (SH)、Hoagland 培养基 (HM)、10X 藻分析程序 (AAP) 和瑞典标准协会培养基 (SIS)) 对浮萍形态生理和生化参数的影响。形态生理参数包括根长、叶片表面积和鲜重，而生化参数包括光合色素、碳水化合物和蛋白质含量。该研究在两个阶段进行了体外实验：无控制通气系统（阶段 I）和控制通气系统（阶段 II）。结果表明，浮萍自然栖息地的 pH 值、电导率、盐度和铵水平均处于最适合浮萍生长的范围内。测量的正磷酸盐浓度与之前的观察结果相比有所升高，而记录的化学需氧量值较低。研究还表明，培养基组成对浮萍的形态生理和生化参数有显著影响。鲜重生物量、叶片相对生长率、表面积相对生长率、根长、蛋白质含量、碳水化合物、叶绿素 (a)、叶绿素 (b)、总叶绿素、类胡萝卜素和叶绿素 (a/b) 比值均受培养基影响。最准确的回归模型描述了两个阶段基于时间和体外培养条件的生长指数 GI(F)。在阶段 I 中，MS、SIS、AAP 和 SH 培养基的最佳模型分别为线性、加权二次、立方和加权立方。在阶段 II 中，所有生长培养基的最佳模型均为线性。阶段 II 的时间系数（以天为单位）分别为 AAP、HM、MS、SH 和 SIS 的 0.321、0.547、1.232、1.470 和 0.306。比较不同培养基中叶片的形态生理和生化参数，并分析回归模型结果表明，在控制通气条件下，SH 和 MS 培养基是测试培养基中最适合浮萍体外培养的培养基。然而，需要进一步研究以开发新的合成培养基，以最好地促进这种浮萍在长期培养中的生长和维持。
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