Department of Cardiovascular Surgery, University of Health Sciences Turkey, Sisli Hamidiye Etfal Training and Research Hospital, Istanbul, Turkey.
Department of Cardiovascular Surgery, Avcılar Hospital, Istanbul, Turkey.
Curr Vasc Pharmacol. 2023;21(4):268-273. doi: 10.2174/1570161121666230502141044.
This study aims to determine the protective effect of cilostazol on myocardium in obese Wistar rats with induced ischemia-reperfusion injury (IRI).
Four groups with 10 Wistar rats were included: 1] Sham Group: IRI was not established in normal weight-Wistar rats. 2] Control Group: IRI but no cilostazol in normal weight-Wistar rats. 3] Cilostazol in normal weight-Wistar rats: IRI and cilostazol was administered. 4] Cilostazol in obese- Wistar rats: IRI and cilostazol was administered.
Tissue adenosine triphosphate (ATP) levels were significantly higher and superoxide dismutase (SOD) levels significantly lower in the control group than in the sham group and normal weight cilostazol group (p=0.024 and p=0.003). Fibrinogen levels were 198 mg/dL in the sham group, 204 mg/dL in the control group, and 187 mg/dL in the normal-weight cilostazol group (p=0.046). Additionally, plasminogen activator inhibitor-1 (PAI-1) levels were significantly higher in the control group (p=0.047). The level of ATP was significantly lower in the normal-weight cilostazol group than in the obese group (104 vs 131.2 nmol/g protein, p=0.043). PAI-1 level was 2.4 ng/mL in the normal weight cilostazol group and 3.7 ng/mL in the obese cilostazol group (p=0.029). Normal-weight Wistar rats with cilostazol had significantly better histologic outcomes than the control group and obese Wistar rats (p=0.001 and p=0.001).
Cilostazol has a protective effect on myocardial cells in IRI models by decreasing inflammation. The protective role of cilostazol was reduced in obese Wistar rats compared with normal-weight Wistar rats.
本研究旨在确定西洛他唑对诱导缺血再灌注损伤（IRI）的肥胖 Wistar 大鼠心肌的保护作用。
纳入 4 组共 10 只 Wistar 大鼠：1]假手术组：在正常体重 Wistar 大鼠中不建立 IRI。2]对照组：在正常体重 Wistar 大鼠中建立 IRI 但不给西洛他唑。3]正常体重 Wistar 大鼠中的西洛他唑组：给予 IRI 和西洛他唑。4]肥胖 Wistar 大鼠中的西洛他唑组：给予 IRI 和西洛他唑。
与假手术组和正常体重西洛他唑组相比，对照组组织三磷酸腺苷（ATP）水平显著升高，超氧化物歧化酶（SOD）水平显著降低（p=0.024 和 p=0.003）。纤维蛋白原水平在假手术组为 198mg/dL，在对照组为 204mg/dL，在正常体重西洛他唑组为 187mg/dL（p=0.046）。此外，对照组纤溶酶原激活物抑制剂-1（PAI-1）水平显著升高（p=0.047）。与肥胖组相比，正常体重西洛他唑组的 ATP 水平显著降低（104 比 131.2nmol/g 蛋白，p=0.043）。正常体重西洛他唑组的 PAI-1 水平为 2.4ng/mL，肥胖西洛他唑组为 3.7ng/mL（p=0.029）。与对照组和肥胖 Wistar 大鼠相比，给予西洛他唑的正常体重 Wistar 大鼠的组织学结果明显更好（p=0.001 和 p=0.001）。
西洛他唑通过减少炎症对 IRI 模型中的心肌细胞具有保护作用。与正常体重 Wistar 大鼠相比，肥胖 Wistar 大鼠中西洛他唑的保护作用降低。
