Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, Rajasthan, India.
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Future Microbiol. 2023 Apr;18:323-334. doi: 10.2217/fmb-2022-0107. Epub 2023 May 4.
The present study evaluates the efficacy of bacteriophage therapy for urinary tract infection (UTI) in rats. UTI was established by inoculating (100 μl) at a concentration of 1.5 × 10 CFU/ml per urethra via a cannula in different groups of rats. For treatment, phage cocktails (200 μl) were administered at varying concentrations of 1 × 10 PFU/ml, 1 × 10 PFU/ml and 1 × 10 PFU/ml. The two doses of phage cocktail at the first two concentrations resulted in the cure of UTI. However, the lowest concentration of the phage cocktail warranted more doses to eradicate the causative bacteria. The quantity, frequency and safety of doses could be optimized in a rodent model using the urethral route.
本研究评估了噬菌体疗法治疗大鼠尿路感染（UTI）的疗效。通过在不同组大鼠的尿道中插入套管，以 1.5×10 CFU/ml 的浓度接种（100 μl），建立 UTI。为了进行治疗，以 1×10 PFU/ml、1×10 PFU/ml 和 1×10 PFU/ml 的不同浓度给予噬菌体鸡尾酒（200 μl）。前两种浓度的两种噬菌体鸡尾酒剂量导致 UTI 治愈。然而，最低浓度的噬菌体鸡尾酒需要更多剂量才能根除致病细菌。通过尿道途径在啮齿动物模型中可以优化剂量的数量、频率和安全性。
