Senjo M, Ishibashi T, Terashima T, Inoue Y
Neurosci Lett. 1986 May 6;66(1):39-42. doi: 10.1016/0304-3940(86)90162-x.
The formation of the capillary-glial complex of developing rat brain ranging in age from embryonic day 16 (E16) rats to birth, which occurs at E20, and in the postnatal rats to day 56 (P56) was determined by measuring the horseradish peroxidase (HRP) activity, which was exogenously injected and penetrated within the brain. The activity of glutathione S-transferases (GSTs) which are located exclusively in astroglial cells was also determined. HRP penetrated into and resided within the central nervous system until birth, although after this age the tracers were rarely observed to be included. On the other hand, GST activity was first detected at E18, and after P5 it increased remarkably. The functional significance of astroglial cells located at the blood-brain or blood-ventricle interface is considered, and we suggest that the ontogenetic development of a blood-brain barrier is compatible with the corresponding appearance of GST.
通过测量外源注射并渗透入脑内的辣根过氧化物酶（HRP）活性，确定了从胚胎第16天（E16）大鼠到出生（发生于E20）以及出生后大鼠至第56天（P56）发育中大鼠脑毛细血管 - 神经胶质复合体的形成。还测定了仅位于星形胶质细胞中的谷胱甘肽S - 转移酶（GSTs）的活性。HRP在出生前渗透并存在于中枢神经系统中，不过在此年龄之后很少观察到示踪剂被摄取。另一方面，GST活性在E18首次检测到，P5之后显著增加。考虑了位于血脑或血 - 脑室界面的星形胶质细胞的功能意义，并且我们认为血脑屏障的个体发育与GST的相应出现是相符的。
