203汞及其他发射γ射线的放射性同位素作为犬恶丝虫微丝蚴的标记物。

203Hg and other gamma-emitting radio-isotopes as labels for Dirofilaria immitis microfilariae.

作者信息

Lengemann F W, Grieve R B, Chmielewicz M, Georgi J R

出版信息

Parasitology. 1986 Apr;92 ( Pt 2):451-61. doi: 10.1017/s0031182000064209.

DOI:10.1017/s0031182000064209

Abstract

The in vitro uptake of gamma-emitting radionuclides by microfilariae of Dirofilaria immitis was investigated. Radionuclides tested were 133Ba, 207Bi, 82Br, 109Cd, 51Cr, 60Co, 59Fe, 203Hg, 125I, 54Mn, 32P, 125Sb, 46Sc, 75Se and 65Zn. Only 207Bi, 59Fe, 203Hg, 54Mn and 46Sc showed more than 2% of the available radioactivity to bind to the microfilariae. When tested for retention in vitro only 203Hg showed retention levels approaching 90%. Moreover, when dimethyl-sulphoxide was incorporated into the medium at levels of 1% (v/v) the uptake of 203Hg could be increased by 3-5 times; no other radio-isotope tested responded in this manner. The uptake of 203Hg was directly related to temperature and time of incubation. Mercury, as mercuric chloride, was toxic to the microfilariae and represents an impediment to the incorporation of high levels of 203Hg in microfilariae.

摘要

研究了犬恶丝虫微丝蚴对发射γ射线的放射性核素的体外摄取情况。所测试的放射性核素有133Ba、207Bi、82Br、109Cd、51Cr、60Co、59Fe、203Hg、125I、54Mn、32P、125Sb、46Sc、75Se和65Zn。只有207Bi、59Fe、203Hg、54Mn和46Sc显示出超过2%的可用放射性与微丝蚴结合。在体外测试保留情况时，只有203Hg的保留水平接近90%。此外，当二甲基亚砜以1%（v/v）的水平加入培养基中时，203Hg的摄取量可增加3至5倍；所测试的其他放射性同位素均未出现这种反应。203Hg的摄取与温度和孵育时间直接相关。汞以氯化汞的形式存在时，对微丝蚴有毒性，这对在微丝蚴中掺入高水平的203Hg构成了障碍。