College of Animal Science, Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, China.
Insect Sci. 2023 Dec;30(6):1595-1606. doi: 10.1111/1744-7917.13200. Epub 2023 May 5.
Fatty acid binding proteins (FABPs) play an important role as endogenous cytoprotectants. However, studies on FABPs in invertebrates are scarce. Previously, we discovered Bombyx mori fatty acid binding protein 1 (BmFABP1) through co-immunoprecipitation. Here, we cloned and identified BmFABP1 from BmN cells. The results of immunofluorescence indicated that BmFABP1 was localized in the cytoplasm. The tissue expression profile of silkworms showed that BmFABP1 was expressed in all tissues except hemocytes. The expression level of BmFABP1 gradually decreases in BmN cells and B. mori larvae after infection with B. mori nucleopolyhedrovirus (BmNPV). Upregulation of BmFABP1 expression through overexpression or WY14643 treatment significantly inhibited the replication of BmNPV, while downregulation of BmFABP1 expression by RNA interference promoted the replication of BmNPV. The same results were obtained in experiments on silkworm larvae. These results suggest that BmNPV induces BmFABP1 downregulation to promote its proliferation and that BmFABP1 has a potential anti-BmNPV role. This is the first report on the antiviral effect of BmFABP1 in silkworms and provides new insights into the study of the FABP protein family. Also, it is important to study BmNPV resistance in silkworms to breed transgenic silkworms with BmNPV resistance.
脂肪酸结合蛋白（FABPs）作为内源性细胞保护剂发挥着重要作用。然而，关于无脊椎动物 FABPs 的研究很少。此前，我们通过共免疫沉淀法发现了家蚕脂肪酸结合蛋白 1（BmFABP1）。在这里，我们从 BmN 细胞中克隆并鉴定了 BmFABP1。免疫荧光的结果表明，BmFABP1 定位于细胞质中。家蚕组织表达谱显示，BmFABP1 除血细胞外，在家蚕的所有组织中均有表达。家蚕核型多角体病毒（BmNPV）感染后，BmN 细胞和家蚕幼虫中 BmFABP1 的表达水平逐渐降低。通过过表达或 WY14643 处理上调 BmFABP1 的表达水平显著抑制了 BmNPV 的复制，而通过 RNA 干扰下调 BmFABP1 的表达水平则促进了 BmNPV 的复制。在家蚕幼虫实验中也得到了相同的结果。这些结果表明，BmNPV 诱导 BmFABP1 下调以促进其增殖，并且 BmFABP1 具有潜在的抗 BmNPV 作用。这是在家蚕中首次报道 BmFABP1 的抗病毒作用，为 FABP 蛋白家族的研究提供了新的见解。此外，研究家蚕对 BmNPV 的抗性对于培育具有 BmNPV 抗性的转基因家蚕非常重要。
