用于检测肾肾小管液中尿素的简易荧光超微量测定法。
Simple fluorometric ultramicroassay for urea in tubular fluid of the kidney.
作者信息
Leng L, Szanyiová M, Faix S, Várady J, Bod'a K
出版信息
Pflugers Arch. 1986 Jun;406(6):648-50. doi: 10.1007/BF00584035.
Sensitive fluorometric method for the determination of picomole amount of urea in tubular fluid of the kidney is presented. It is a modification and simplification of the macromethod by McCleskey (6) for the urea determination in blood. Assay is based on the reaction of urea with diacetylmonoxime and sulphuric acid in boiling water within 20 minutes. The reaction is highly specific for urea. The simple procedure requires only one micropipetting step. The fluorometric determination of urea can be made in 20 nanoliters of proximal and in 5 nanoliters of distal tubular fluid with the reproducibility of +/- 4.44% and of +/- 5.38%, respectively. The minimum detection limit is 20 picomoles of urea.
本文介绍了一种灵敏的荧光法，用于测定肾肾小管液中皮摩尔量的尿素。它是对麦克莱斯基（6）用于血液中尿素测定的宏观方法的改进和简化。该测定基于尿素与二乙酰一肟和硫酸在沸水中20分钟内的反应。该反应对尿素具有高度特异性。简单的操作程序仅需一个微量移液步骤。可以在20纳升的近端肾小管液和5纳升的远端肾小管液中进行尿素的荧光测定，重现性分别为±4.44%和±5.38%。最低检测限为20皮摩尔尿素。
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