Department of Clinical Laboratory, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250021, Shandong, China.
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 9677# Jing-Shi Road, Jinan, 250014, People's Republic of China.
Int Microbiol. 2023 Nov;26(4):1157-1166. doi: 10.1007/s10123-023-00369-7. Epub 2023 May 5.
Urinary tract infection (UTI) is one of the most common extraintestinal infections, and uropathogenic Escherichia coli (UPEC) is the main cause of UTIs. However, the ability to treat UTI has been compromised by the increase in antimicrobial resistance, especially carbapenem resistance. Here, we aimed to characterize the antimicrobial resistance and molecular epidemiology of carbapenem-resistant UPEC isolated in Shandong, China.
In total, 17 carbapenem-resistant UPEC (CR-UPEC) isolates were collected from July 2017 to May 2020 in the Shandong Provincial Hospital. Whole-genome sequencing and bioinformatics analyses were performed to understand the molecular epidemiology of CR-UPEC. Phylogenetic groups, drug resistance genes, biofilm formation, and virulence-related gene profiles of the isolates were analyzed. Plasmid profiling and conjugation assay were performed to evaluate the ability to transfer carbapenem resistance-related genes to other E. coli isolates. Biofilm formation was also evaluated, as it is important for the persistence of infectious diseases.
We observed that 15 out of 17 CR-UPEC strains were bla producers, among which 4 isolates could transfer bla to recipient cells. The predominant sequence type was ST167 (6/17), followed by ST410 (3/17). The most prevalent phylogenetic group was phylogenetic group A (10/17), followed by phylogenetic group C (3/17). One isolate was resistant to polymyxin, which was caused by the carriage of a transferable plasmid harboring mcr-1. Statistical analysis did not reveal any significant difference in the carriage rate of fimbriae-coding genes between strong and weak biofilm producers.
Our observations may assist in developing new therapeutic methods for drug-resistant organisms.
尿路感染（UTI）是最常见的肠道外感染之一，而尿路致病性大肠杆菌（UPEC）是 UTIs 的主要原因。然而，抗菌药物耐药性的增加，特别是碳青霉烯类耐药性的增加，已经削弱了治疗 UTI 的能力。在这里，我们旨在描述中国山东分离的耐碳青霉烯类 UPEC 的抗菌药物耐药性和分子流行病学特征。
从 2017 年 7 月至 2020 年 5 月，我们从山东省医院共收集了 17 株耐碳青霉烯类 UPEC（CR-UPEC）分离株。我们进行了全基因组测序和生物信息学分析，以了解 CR-UPEC 的分子流行病学。分析了分离株的进化群、耐药基因、生物膜形成和与毒力相关的基因谱。进行了质粒图谱分析和接合试验，以评估将碳青霉烯类耐药相关基因转移到其他大肠杆菌分离株的能力。还评估了生物膜形成，因为它对传染病的持续存在很重要。
我们观察到 17 株 CR-UPEC 中有 15 株是 bla 基因的产生者，其中 4 株可以将 bla 基因转移到受体细胞。主要的序列类型是 ST167（17 株中的 6 株），其次是 ST410（17 株中的 3 株）。最常见的进化群是 A 群（17 株中的 10 株），其次是 C 群（17 株中的 3 株）。有 1 株对多黏菌素耐药，这是由于携带 mcr-1 的可转移质粒引起的。统计学分析没有显示出强生物膜形成者和弱生物膜形成者携带菌毛编码基因的携带率有任何显著差异。
我们的观察结果可能有助于为耐药菌开发新的治疗方法。
