Department of Pathology, Maulana Azad Medical College, New Delhi, India.
Department of Surgery, Maulana Azad Medical College, New Delhi, India.
J Cancer Res Ther. 2023 Apr;19(Supplement):S172-S176. doi: 10.4103/jcrt.jcrt_770_22.
Colorectal carcinoma (CRC) is third most common malignancy in the world. The presence of Lymphocytes particularly at the invasive margin of the tumor have been associated with good immune response indicating better prognosis. The relative tumor stroma is also important in deciding the course of the disease. The Glasgow Microenvironment Score (GMS) comprises of assessment of tumor cell infiltrate using Klintrup-Makinen (KM) grade and tumor stroma percentage.
The aim of the present study is to evaluate the utility of GMS score in relation to parameters of adverse histopathological outcome in carcinoma colon that is grading, staging, LVI, PNI and nodal metastasis.
Colectomy specimens received over 3 year period were subjected to microscopic evaluation for LVI, PNI, grade, stage & lymph node metastasis.
Lymphocytes at the deepest invasive margin of tumor were counted as per KM score in 5 HPF by two independent pathologists . Patients were classified as Low grade (0/1) or high grade (2/3) response. Tumor stroma percentage was calculated as stroma poor (<50%) and stroma rich (>/= 50 %). The GMS was scored by combining the two and scored 0, 1 and 2.
Total 37 patients with no prior therapy were included out of which 23 were males and 14 females. 15 patients (40.54%) had GMS of 0, 6 (16.21%) had GMS 1 and 16 (43.24%) had GMS 2. A high GMS was associated with LVI (P = 0.02), PNI (P = 0.01) lymph node metastasis (P = 0.003). However, no significant association was found between GMS with Grade (P = 0.98) and Stage (P = 0.36).
Low GMS was associated with good outcome and high GMS with poor outcome. This score can be used for risk stratification, can be of clinical utility and may be applied to pathological descriptions of CRC.
结直肠癌（CRC）是世界上第三大常见恶性肿瘤。淋巴细胞的存在，特别是在肿瘤的浸润边缘，与良好的免疫反应相关，表明预后较好。相对肿瘤间质在决定疾病进程方面也很重要。格拉斯哥微环境评分（GMS）包括使用克林特鲁普-马金宁（KM）分级和肿瘤间质百分比评估肿瘤细胞浸润。
本研究旨在评估 GMS 评分与结肠癌不良组织病理学结局参数的关系，这些参数包括分级、分期、LVI、PNI 和淋巴结转移。
在 3 年期间收到的结肠切除术标本进行了 LVI、PNI、分级、分期和淋巴结转移的显微镜评估。
两名独立的病理学家在 5HPF 中根据 KM 评分对肿瘤最深浸润边缘的淋巴细胞进行计数。患者被分为低级别（0/1）或高级别（2/3）反应。计算肿瘤间质百分比，判断为间质不良（<50%）和间质丰富（≥50%）。通过结合这两个评分，将 GMS 评分分为 0、1 和 2。
共纳入 37 例未经治疗的患者，其中 23 例为男性，14 例为女性。15 例（40.54%）患者的 GMS 评分为 0，6 例（16.21%）患者的 GMS 评分为 1，16 例（43.24%）患者的 GMS 评分为 2。高 GMS 与 LVI（P=0.02）、PNI（P=0.01）和淋巴结转移（P=0.003）相关。然而，GMS 与分级（P=0.98）和分期（P=0.36）之间无显著相关性。
低 GMS 与良好的预后相关，而高 GMS 与不良的预后相关。该评分可用于风险分层，具有临床实用性，并可应用于 CRC 的病理描述。
