Department of Biochemistry, Andhra University, Visakhapatnam, Andhra Pradesh, India.
J Cancer Res Ther. 2023 Apr;19(Supplement):S241-S249. doi: 10.4103/jcrt.JCRT_160_18.
Nymphaea nouchali Brum is exotic and medicinal plant in India.
The main of this study is to evaluate the anticancer properties of Nymphaea nouchali Brum flowers against Ehrlich ascites carcinoma (EAC)-induced Swiss albino mice.
The anticancer properties of Nymphaea nouchali Brum dry and fresh methanol extracts was investigated using EAC in Swiss albino mice. After inoculation of EAC cells into mice, treatment with NNDM flower extract (200 and 400 mg/kg) and standard drug 5-Fluorouracil (20 mg/kg) was continued for 9 days. The evaluation of the effect of drug response was made by the study of tumor growth response including increase in lifespan, the study of hematological parameters, biochemical estimations, and antioxidant assay of liver tissue compared to EAC control. The viability of cancer cell lines (such as HeLa, MCF-7, and MDA-MB 231 cells) was evaluated by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay.
Therefore, from the results of the present study, it can be concluded that NNDM exhibited significant antitumor activity against EAC in Swiss albino mice. The effect of NNDM on viability of cancer cell lines (such as HeLa, MCF-7, and MDA-MB 231 cells) was evaluated by MTT assay, apoptosis in HeLa cell lines was evaluated by DNA laddering assay, HeLa cells treated with NNDM exhibited a characteristic "ladder" pattern after separation of the fragments by agarose gel electrophoresis and subsequent visualization, by ethidium bromide staining. NNDM exhibited a significant effect on cell viability.
Based on results, it was concluded that NNDM exhibited cytotoxic effect on cancer cells and, from DNA laddering assay, it can be concluded that NNDM-induced apoptosis in EAC cells.
芡实属印度的外来药用植物。
本研究的主要目的是评估芡鲜花对艾氏腹水癌（EAC）诱导的瑞士白化病小鼠的抗癌特性。
采用 EAC 对瑞士白化病小鼠研究芡干甲醇提取物和新鲜甲醇提取物的抗癌特性。EAC 细胞接种后，继续用 NNDM 花提取物（200 和 400mg/kg）和标准药物 5-氟尿嘧啶（20mg/kg）治疗 9 天。通过研究肿瘤生长反应（包括寿命延长）、血液学参数研究、肝组织生化估计和抗氧化测定来评估药物反应的效果，与 EAC 对照组进行比较。通过 3-（4,5-二甲基噻唑基-2）-2,5-二苯基四氮唑溴化物（MTT）测定评估癌症细胞系（如 HeLa、MCF-7 和 MDA-MB 231 细胞）的细胞活力。
因此，从本研究的结果可以得出结论，NNDM 对瑞士白化病小鼠的 EAC 表现出显著的抗肿瘤活性。通过 MTT 测定评估 NNDM 对癌症细胞系（如 HeLa、MCF-7 和 MDA-MB 231 细胞）活力的影响，通过 DNA 梯状电泳测定评估 HeLa 细胞系中的细胞凋亡，HeLa 细胞用 NNDM 处理后，琼脂糖凝胶电泳分离片段后，通过溴化乙锭染色，观察到特征性的“梯状”模式。NNDM 对细胞活力有显著影响。
基于结果，可以得出结论，NNDM 对癌细胞表现出细胞毒性作用，并且从 DNA 梯状电泳测定可以得出结论，NNDM 诱导 EAC 细胞凋亡。
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