Antitumour activity of Annona muricata L. leaf methanol extracts against Ehrlich Ascites Carcinoma and Dalton's Lymphoma Ascites mediated tumours in Swiss albino mice.

机构信息

Faculty of Life Sciences & Environment, Department of Botany, Goa University , Taleigao Plateau, India.

Physiology and Biochemistry Laboratory, Department of Zoology, Goa University , Taleigao Plateau, India.

出版信息

Libyan J Med. 2021 Dec;16(1):1846862. doi: 10.1080/19932820.2020.1846862.

The use of plants as a source of sedative or treatment for cancer is reasonably widespread worldwide. Linn exhibits a vast array of medicinal and ethno-pharmaceutical benefits, attributed by different plant parts. The activity of this plant is regarded to the bio-production of secondary metabolites like alkaloids, phenols, flavonoids, and most unique group of compounds, namely, annonaceous acetogenins. Whilst this plant is gaining popularity as an anticancer treating plant, this study was undertaken to verify the plausible anticancer effect of leaf methanol extracts of (LEAM). Acute toxicity study was carried to obtain safe dose in mice models using haematological, biochemical, and histological evaluations in Swiss albino mice. cytotoxicity towards Dalton's Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma (EAC) cell lines were determined by trypan blue exclusion method. antitumour activity of LEAM (100, 200, and 500mg/kg b.wt.) was evaluated using DLA induced solid carcinoma and EAC induced ascites carcinoma models and its comparison with standard drug Cisplatin. Acute toxicity studies did not exhibit significant variations in treated mice suggesting diminutive side effects of LEAM. Statistical analysis revealed the IC values for DLA and EAC cell lines as 85.56 ± 5.28 and 68.07 ± 7.39 µg/mL, respectively, indicating better cytotoxic activity against EAC than DLA cells. LEAM decreased the tumour burden in dose-dependent manner. In comparison, with different concentrations tested, treatment with LEAM (200 mg/kg b.wt. and 500 mg/kg b.wt.) significantly reduced the solid tumour volume development by 58.11% and 65.70%, respectively. While lifespan was prolonged up to 51.43% in 500 mg/kg b.wt. LEAM treated ascites tumour-induced mice. This study thus indicates that LEAM possesses potent cytotoxic and antineoplastic activity and calls for more methodical safety assessments and other end-points of anti-tumourigenesis. : : Leaf methanol extract of muricata; : Dalton's Lymphoma Ascites; : Ehrlich Ascites Carcinoma; : Half maximal inhibitory concentration; : Committee for the Purpose of Control Supervision of Experiments on Animal; : Institutional Animal Ethics Committee; : Animal Research: Reporting Experiments; : Dimethyl sulphoxide; : Lethal Dose, 50%; : Standard Deviation; : Haemoglobin; : Red blood cells; : White blood cells; : Hematocrit; : Mean cell volume; : Mean cell haemoglobin; : Mean cell haemoglobin concentration; : Serum alkaline phosphatase; : Serum glutamic pyruvic transaminase; : Serum glutamic oxaloacetic transaminase; : Adenosine triphosphate; : Epidermal Growth Factor Receptor.

作为镇静剂或癌症治疗药物来源，植物的使用在全球范围内相当普遍。 Linn 表现出广泛的药用和民族药理学益处，归因于不同的植物部位。该植物的活性被认为是生物产生生物碱、酚类、类黄酮和最独特的一类化合物，即番荔枝乙酰基生物类。虽然这种植物作为抗癌治疗植物越来越受欢迎，但进行这项研究是为了验证 Linn 叶甲醇提取物 (LEAM) 的合理抗癌作用。通过对瑞士白化小鼠进行血液学、生物化学和组织学评估，进行急性毒性研究以获得小鼠模型中的安全剂量。使用台盼蓝排除法测定对 Dalton's Lymphoma Ascites (DLA) 和 Ehrlich Ascites Carcinoma (EAC) 细胞系的细胞毒性。通过 DLA 诱导的实体癌和 EAC 诱导的腹水癌模型评估 LEAM（100、200 和 500mg/kg b.wt.）的抗肿瘤活性，并与标准药物顺铂进行比较。急性毒性研究未显示治疗小鼠有显著变化，表明 LEAM 的副作用极小。统计分析显示 DLA 和 EAC 细胞系的 IC 值分别为 85.56±5.28 和 68.07±7.39μg/mL，表明对 EAC 的细胞毒性活性优于 DLA 细胞。LEAM 以剂量依赖的方式降低肿瘤负担。相比之下，用不同浓度测试时，LEAM（200mg/kg b.wt. 和 500mg/kg b.wt.）治疗可使实体肿瘤体积分别减少 58.11%和 65.70%。而在 500mg/kg b.wt.LEAM 治疗的腹水瘤诱导小鼠中，寿命延长至 51.43%。因此，这项研究表明 LEAM 具有强大的细胞毒性和抗肿瘤活性，并呼吁进行更系统的安全性评估和其他抗肿瘤发生的终点研究。 Linn muricata Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn

解析

这段文本是关于 Linn muricata（ Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn Linn In vivo cytotoxicity study showed that LEAM treatment decreased the tumor burden in a dose-dependent manner. In comparison, with different concentrations tested, treatment with LEAM (200 mg/kg b.wt. and 500 mg/kg b.wt.) significantly reduced the solid tumor volume development by 58.11% and 65.70%, respectively. While lifespan was prolonged up to 51.43% in 500 mg/kg b.wt. LEAM treated ascites tumor-induced mice. This study thus indicates that LEAM possesses potent cytotoxic and antineoplastic activity and calls for more methodical safety assessments and other end-points of anti-tumourigenesis.