RPTU Institute of Environmental Sciences, Landau, Fortstrasse 7, 76829 Landau, Germany.
Leibniz Institute of Ecological Urban and Regional Development (IOER), Dresden, Germany.
Chemosphere. 2023 Aug;331:138840. doi: 10.1016/j.chemosphere.2023.138840. Epub 2023 May 4.
A simple acetonitrile-based extraction method for the determination of 98 current-use pesticides (CUPs) in soil and herbaceous vegetation using HPLC-ESI-MS/MS is reported. The method was optimized in terms of extraction time, buffer (ammonium formate) ratio, and graphitized carbon black (GCB) ratio for the clean-up of vegetation. The validated method yielded accuracy in terms of percentage recovery of 71-125% (soil) and 70-117% (vegetation) for the majority of 98 CUPs. The precision in terms of relative standard deviation was at 1-14% (soil), and 1-13% (vegetation). Matrix-matched calibration curves exhibited good linearities (R > 0.99). The limits of quantitation ranged between 0.008 and 21.5 μg kg in soil and vegetation. The reported method was applied to soils and vegetation from 13 agricultural sites across Germany. Overall, 44 of the 98 common CUPs were detected in our samples and the qualitative load is well above the average for arable soils in the EU.
本文报道了一种基于乙腈的简单提取方法，用于使用 HPLC-ESI-MS/MS 测定土壤和草本植物中的 98 种当前使用的农药（CUPs）。该方法在提取时间、缓冲液（甲酸铵）比例和用于植物净化的石墨化碳黑（GCB）比例方面进行了优化。经验证，该方法对于大多数 98 种 CUPs 的回收率为 71-125%（土壤）和 70-117%（植物），具有良好的准确性。相对标准偏差的精密度为 1-14%（土壤）和 1-13%（植物）。基质匹配校准曲线表现出良好的线性（R>0.99）。定量限在土壤和植物中的范围分别为 0.008 至 21.5μgkg。所报道的方法应用于德国 13 个农业地点的土壤和植被。总体而言，在我们的样本中检测到了 98 种常见 CUPs 中的 44 种，其定性负荷明显高于欧盟耕地土壤的平均水平。
