Zanin L G, Schmitt D, Ohrt C, Thivolet J
Ann Dermatol Venereol. 1978 Oct;105(10):793-8.
A comparative study of the sensitivities of the immunofluorescence (IF) and immunoperoxydase (IPo) techniques was undertaken. The material used was biopsies of various skin lesions and especially pemphigus, bullous pemphigoid, lupus erythematosus and dermatitis herpetiformis. Direct and indirect methods were performed for the detection of fixed immunoglobulins and complement deposits. With large deposits, immunoperoxydase and immunofluorescence show an equal sensibility and the labeling obtained with IPo is never more intensive than the labeling obtained with IF. In contrast, with small deposits, IPo was less sensitive than IF and appears negative in the majority of the cases and especially in dermatitis herpetiformis. These results are confirmed in using increasing dilutions of conjugates and can be explained by the difficulty in doing the observation of small positive deposits with IPo.
对免疫荧光（IF）和免疫过氧化物酶（IPo）技术的敏感性进行了一项比较研究。所用材料为各种皮肤病变的活检组织，尤其是天疱疮、大疱性类天疱疮、红斑狼疮和疱疹样皮炎。采用直接和间接方法检测固定的免疫球蛋白和补体沉积。对于大量沉积物，免疫过氧化物酶和免疫荧光显示出相同的敏感性，并且IPo获得的标记强度绝不会比IF获得的标记更强。相比之下，对于少量沉积物，IPo比IF敏感性低，并且在大多数病例中呈阴性，尤其是在疱疹样皮炎中。在使用逐渐稀释的结合物时证实了这些结果，并且可以通过用IPo观察小的阳性沉积物存在困难来解释。
