Laboratory of Yeast Biology and Fermentation Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China.
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Protein Expr Purif. 2023 Aug;208-209:106294. doi: 10.1016/j.pep.2023.106294. Epub 2023 May 5.
Chitin is a polymer of β-1,4-linked N-acetylglucosamine (GlcNAc) and plays a central role in the assembly of the fungal cell wall. Chitinases are hydrolytic enzymes that break down glycosidic bonds in the chitin. Chitinases are classified into three categories, endochitinases, exochitinases and N-acetylglucosaminases, according to the manner in which the enzyme cleaves the chitin polymer. Saccharomyces cerevisiae has two chitinase-encoding genes, CTS1 and CTS2. However, whether Cts2p shows a chitinase activity remains unknown. In this study, we have cloned, expressed and purified the recombinant Cts2p protein from bacterial cells. We have demonstrated that Cts2p has a higher chitobiosidase (exochitinase) activity than endochitinase activity, but no N-acetylglucosaminase activity. The optimal temperature for the chitobiosidase activity of Cts2p is 37 °C.
几丁质是由β-1,4 键连接的 N-乙酰葡萄糖胺（GlcNAc）组成的聚合物，在真菌细胞壁的组装中起核心作用。几丁质酶是水解酶，可分解几丁质中的糖苷键。根据酶切割几丁质聚合物的方式，几丁质酶可分为内切几丁质酶、外切几丁质酶和 N-乙酰葡萄糖胺酶三类。酿酒酵母有两个几丁质酶编码基因，CTS1 和 CTS2。然而，Cts2p 是否具有几丁质酶活性尚不清楚。在本研究中，我们从细菌细胞中克隆、表达和纯化了重组 Cts2p 蛋白。结果表明，Cts2p 具有较高的壳二糖酶（外切几丁质酶）活性，而内切几丁质酶活性较低，但没有 N-乙酰葡萄糖胺酶活性。Cts2p 壳二糖酶活性的最佳温度为 37°C。
