AlSunbul Hanan, Murriky Afraa
Department of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Department of Restorative Dentistry, College of Dentistry, Riyadh Elm University, Riyadh, Saudi Arabia.
Photodiagnosis Photodyn Ther. 2023 Jun;42:103598. doi: 10.1016/j.pdpdt.2023.103598. Epub 2023 May 6.
This study aimed to evaluate the adhesive bond strength and antibacterial efficacy of methylene blue (MB)-mediated antimicrobial photodynamic therapy (aPDT) and curcumin (CUR)-mediated aPDT versus the conventional disinfectants, such as chlorhexidine gluconate (CHX) gel and sodium hypochlorite (NaOCl), for indirect pulp capping (IPC) treatment of permanent molars.
One Hundred grossly carious human permanent molars were collected through non-traumatic extraction. All specimens were embedded in polyvinyl cross-sections to the cemento-enamel junction. The cavity preparation was conducted by grinding the samples using silicon carbide discs. After culturing Streptococcus mutans (S. mutans), a 10 µL of S. mutans suspension (10 colony forming units/mL) was transferred in each tooth cavity and anaerobically incubated for 48 h at 37 °C. All specimens were randomly divided into 5 groups: Group-I: samples treated IPC; Group-II: samples treated with 2% CHX gel; Group-III: samples treated with 6% NaOCl; Group-IV: irradiation of prepared cavity with MB-mediated aPDT; and Group-V: irradiation of prepared cavity with CUR-mediated aPDT. After disinfection methods, the universal adhesive was used, and all specimens were restored using giomer. Eventually, confocal laser scanning microscopy, shear bond strength (SBS), micro-tensile bond strength (μTBS), four-point bending strength (4P-BS) analyses were performed, and the data were analyzed statistically.
At baseline, the highest SBS (48.8 ± 6.5 MPa), μTBS (54.3 ± 3.9 MPa), and 4P-BS (123 ± 32 MPa) scores were demonstrated by the samples treated with MB-mediated aPDT. However, after 12 months of storage, the highest SBS (42.3 ± 3.9 MPa) and μTBS (45.2 ± 6.6 MPa) scores were shown by samples treated with MB-mediated aPDT, while CUR-mediated aPDT treated samples demonstrated the highest 4P-BS scores (70 ± 18 MPa). Moreover, the highest antibacterial activity against S. mutans was shown by the samples treated with MB-mediated aPDT.
The application of aPDT, especially MB-mediated, demonstrated superior SBS, μTBS, and 4P-BS values as well as antibacterial activity against S. mutans as compared to 2% CHX gel and 6% NaOCl as cavity disinfectants for IPC treatment of permanent molars.
本研究旨在评估亚甲蓝（MB）介导的抗菌光动力疗法（aPDT）和姜黄素（CUR）介导的aPDT与传统消毒剂（如葡萄糖酸氯己定（CHX）凝胶和次氯酸钠（NaOCl））相比，用于恒牙间接盖髓术（IPC）治疗时的粘结强度和抗菌效果。
通过非创伤性拔牙收集100颗严重龋坏的人类恒牙。将所有标本嵌入到聚乙烯中，制成至牙骨质-釉质界的横截面。使用碳化硅盘研磨样本进行窝洞制备。培养变形链球菌（S. mutans）后，将10μL变形链球菌悬液（10个菌落形成单位/mL）转移到每个牙洞中，并在37℃下厌氧培养48小时。所有标本随机分为5组：第一组：接受IPC治疗的样本；第二组：用2% CHX凝胶治疗的样本；第三组：用6% NaOCl治疗的样本；第四组：用MB介导的aPDT照射制备好的窝洞；第五组：用CUR介导的aPDT照射制备好的窝洞。消毒处理后，使用通用粘结剂，所有标本用聚酸改性复合树脂修复。最后，进行共聚焦激光扫描显微镜检查、剪切粘结强度（SBS）、微拉伸粘结强度（μTBS）、四点弯曲强度（4P-BS）分析，并对数据进行统计学分析。
在基线时，用MB介导的aPDT处理的样本显示出最高的SBS（48.8±6.5MPa）、μTBS（54.3±3.9MPa）和4P-BS（123±32MPa）评分。然而，储存12个月后，用MB介导的aPDT处理的样本显示出最高的SBS（42.3±3.9MPa）和μTBS（45.2±6.6MPa）评分，而用CUR介导的aPDT处理的样本显示出最高的4P-BS评分（70±18MPa）。此外，用MB介导的aPDT处理的样本对变形链球菌显示出最高的抗菌活性。
与2% CHX凝胶和6% NaOCl作为恒牙IPC治疗的窝洞消毒剂相比，aPDT的应用，尤其是MB介导的aPDT，显示出更高的SBS、μTBS和4P-BS值以及对变形链球菌的抗菌活性。
