Saunders G K
Res Commun Chem Pathol Pharmacol. 1986 Apr;52(1):45-9.
Fifteen male Sprague-Dawley rats (250 g - 300 g) were randomly divided into 5 control and 10 diabetic. Diabetes was induced by streptozotocin injection. Five diabetic rats were treated with penicillamine to chelate copper. All rats were anesthetized for renal clearance studies three weeks following streptozotocin injection. Inulin, sodium p-aminohippurate (PAH) and dextrose were infused i.v. and serial blood and urine samples collected for inulin, PAH and glucose analysis. Renal copper was determined following euthanasia. The glomerular filtration rate (GFR) and tubular maximum (Tm) for PAH and glucose were calculated. These values and the renal copper levels were compared between the three groups using the student's t-test. GFR and the Tm for PAH and glucose were increased in both diabetic groups as compared to the control group (p less than .05), but no difference was noted between the two diabetic groups. Renal copper levels were increased in the non-treated diabetic rats but not in the penicillamine-treated rats (p less than .01) indicating that penicillamine effectively removed copper from the diabetic kidney. The conclusion is that excess renal copper in the diabetic rat does not affect renal function in the early stages of diabetes.
将15只雄性Sprague-Dawley大鼠（250克 - 300克）随机分为5只对照组和10只糖尿病组。通过注射链脲佐菌素诱导糖尿病。5只糖尿病大鼠用青霉胺治疗以螯合铜。在注射链脲佐菌素三周后，所有大鼠均麻醉以进行肾清除率研究。静脉输注菊粉、对氨基马尿酸钠（PAH）和葡萄糖，并采集系列血液和尿液样本用于菊粉、PAH和葡萄糖分析。安乐死后测定肾铜含量。计算PAH和葡萄糖的肾小球滤过率（GFR）和肾小管最大分泌量（Tm）。使用学生t检验比较三组之间的这些值和肾铜水平。与对照组相比，两个糖尿病组的GFR以及PAH和葡萄糖的Tm均升高（p小于0.05），但两个糖尿病组之间未观察到差异。未治疗的糖尿病大鼠肾铜水平升高，但青霉胺治疗的大鼠未升高（p小于0.01），表明青霉胺有效地从糖尿病肾脏中去除了铜。结论是糖尿病大鼠肾内过量的铜在糖尿病早期不影响肾功能。
