Pan Jia-Yuan, Wang Chao-Bei, Nong Jie-Liang, Xie Qing-Lin, Shen Tai-Ming
School of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541004 People's Republic of China.
School of Energy and Building Environment, Guilin University of Aerospace Technology, Guilin, 541004 People's Republic of China.
3 Biotech. 2023 May;13(5):158. doi: 10.1007/s13205-023-03593-0. Epub 2023 May 3.
The difficulty of releasing nutrients from soils in karst areas limits the yield of local crops and leads to poverty. In this study, two strains of plant growth-promoting rhizobacteria (PGPR) were isolated from the rhizosphere soil of typical plants in karst areas, which were both identified as sp. and named GS1 and N1. And two isolates were used to construct a composite PGPR named MC1. These three strains of PGPR were used for soil inoculation in the pot experiment and field trial and their capacity to promote rice development was assessed. The results showed that MC1 inoculation exhibited notable rice growth-promoting ability in pot experiments, and, respectively, had an increment of 16.96, 18.74, and 11.50% in shoot biomass, total biomass, and rice height compared with control. This is largely attributed to PGPR's capacity to secrete phytohormones and soil enzymes, particularly urease (UE) in GS1, whose secreted UE content was significantly higher by 12.18% compared to the control. When applied to the field, MC1 inoculation not only increased rice yield by 8.52% and the available nutrient content in rice rhizosphere soil, such as available phosphorus (AP) and exchangeable magnesium (EMg); but also improved the abundance of beneficial rhizobacteria and the diversity of microbial communities in rice rhizosphere soil. Results in this study revealed that inoculated PGPR played a major role in promoting rice growth and development, and a new strategy for facilitating the growth of rice crops in agriculture was elucidated.
The online version contains supplementary material available at 10.1007/s13205-023-03593-0.
未标注：喀斯特地区土壤养分释放困难，限制了当地农作物产量，导致贫困。本研究从喀斯特地区典型植物的根际土壤中分离出两株植物促生根际细菌（PGPR），均鉴定为 属，命名为GS1和N1。将这两株分离物用于构建复合PGPR，命名为MC1。将这三株PGPR用于盆栽试验和田间试验的土壤接种，并评估它们促进水稻生长的能力。结果表明，在盆栽试验中，接种MC1表现出显著的促进水稻生长的能力，与对照相比，地上部生物量、总生物量和水稻株高分别增加了16.96%、18.74%和11.50%。这在很大程度上归因于PGPR分泌植物激素和土壤酶的能力，特别是GS1中的脲酶（UE），其分泌的UE含量比对照显著高出12.18%。在田间应用时，接种MC1不仅使水稻产量提高了8.52%，还提高了水稻根际土壤中有效养分的含量，如有效磷（AP）和交换性镁（EMg）；同时还提高了水稻根际土壤中有益根际细菌的丰度和微生物群落的多样性。本研究结果表明，接种PGPR在促进水稻生长发育中起主要作用，并阐明了一种促进水稻作物生长的新策略。
在线版本包含可在10.1007/s13205-023-03593-0获取的补充材料。
