GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macau Joint Laboratory for Cell Fate Regulation and Diseases, The State Key Lab of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, China.
Front Cell Infect Microbiol. 2023 Apr 19;13:1170505. doi: 10.3389/fcimb.2023.1170505. eCollection 2023.
Low temperature is conducive to the survival of COVID-19. Some studies suggest that cold-chain environment may prolong the survival of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and increase the risk of transmission. However, the effect of cold-chain environmental factors and packaging materials on SARS-CoV-2 stability remains unclear.
This study aimed to reveal cold-chain environmental factors that preserve the stability of SARS-CoV-2 and further explore effective disinfection measures for SARS-CoV-2 in the cold-chain environment. The decay rate of SARS-CoV-2 pseudovirus in the cold-chain environment, on various types of packaging material surfaces, i.e., polyethylene plastic, stainless steel, Teflon and cardboard, and in frozen seawater was investigated. The influence of visible light (wavelength 450 nm-780 nm) and airflow on the stability of SARS-CoV-2 pseudovirus at -18°C was subsequently assessed.
Experimental data show that SARS-CoV-2 pseudovirus decayed more rapidly on porous cardboard surfaces than on nonporous surfaces, including polyethylene (PE) plastic, stainless steel, and Teflon. Compared with that at 25°C, the decay rate of SARS-CoV-2 pseudovirus was significantly lower at low temperatures. Seawater preserved viral stability both at -18°C and with repeated freeze-thaw cycles compared with that in deionized water. Visible light from light-emitting diode (LED) illumination and airflow at -18°C reduced SARS-CoV-2 pseudovirus stability.
Our studies indicate that temperature and seawater in the cold chain are risk factors for SARS-CoV-2 transmission, and LED visible light irradiation and increased airflow may be used as disinfection measures for SARS-CoV-2 in the cold-chain environment.
低温有利于 COVID-19 的存活。一些研究表明，冷链环境可能会延长严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的存活时间，增加传播风险。然而，冷链环境因素和包装材料对 SARS-CoV-2 稳定性的影响尚不清楚。
本研究旨在揭示保存 SARS-CoV-2 稳定性的冷链环境因素，并进一步探索冷链环境中 SARS-CoV-2 的有效消毒措施。研究了 SARS-CoV-2 假病毒在冷链环境中、在不同类型的包装材料表面（聚乙烯塑料、不锈钢、特氟龙和纸板）以及冷冻海水中的衰减率。随后评估了可见光（波长 450nm-780nm）和气流对-18°C 下 SARS-CoV-2 假病毒稳定性的影响。
实验数据表明，SARS-CoV-2 假病毒在多孔纸板表面的衰减速度比在非多孔表面（包括聚乙烯（PE）塑料、不锈钢和特氟龙）上更快。与 25°C 相比，SARS-CoV-2 假病毒在低温下的衰减速度明显较低。与去离子水相比，海水在-18°C 及反复冻融循环中均能保持病毒稳定性。-18°C 下发光二极管（LED）照明产生的可见光和气流降低了 SARS-CoV-2 假病毒的稳定性。
我们的研究表明，冷链中的温度和海水是 SARS-CoV-2 传播的风险因素，LED 可见光照射和增加气流可能可作为冷链环境中 SARS-CoV-2 的消毒措施。
Zotero 插件
