Chen Guo-Rong, Zhang Yi-Bin, Zheng Shu-Fa, Xu Ya-Wen, Lin Peng, Shang-Guan Huang-Cheng, Lin Yuan-Xiang, Kang De-Zhi, Yao Pei-Sen
Department of Neurosurgery, Neurosurgical Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350005, P.R. China.
Department of Neurosurgery, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350212, P.R. China.
Exp Ther Med. 2023 Apr 18;25(6):253. doi: 10.3892/etm.2023.11952. eCollection 2023 Jun.
The majority of low-grade gliomas (LGGs) in adults invariably progress to glioblastoma over time. Spectrin β non-erythrocytic 2 () is detected in numerous tumors and is involved in tumor occurrence and metastasis. However, the specific roles and detailed mechanisms of in LGG are largely unknown. The present study performed pan-cancer analysis for the expression and prognosis of SPTBN2 in LGG using The Cancer Genome Atlas and The Genotype-Tissue Expression. Western blotting was used to detect the amount of SPTBN2 between glioma tissues and normal brain tissues. Subsequently, based on expression, prognosis, correlation and immune infiltration, non-coding RNAs (ncRNAs) were identified that regulated expression. Finally, tumor immune infiltrates associated with and prognosis were performed. Lower expression of was correlated with an unfavorable outcome in LGG. A significant correlation between the low mRNA expression and poor clinicopathological features was observed, including wild-type isocitrate dehydrogenase status (P<0.001), 1p/19q non-codeletion (P<0.001) and elders (P=0.019). The western blotting results revealed that, compared with normal brain tissues, the amount of SPTBN2 was significantly lower in LGG tissues (P=0.0266). Higher expression of five microRNAs (miRs/miRNAs), including and , correlated with poor prognosis by targeting SPTBN2 in LGG. Subsequently, four long ncRNAs (lncRNAs) [antisense RNA 1 ] were observed in the regulation of via five miRNAs. Moreover, the expression of was significantly correlated with tumor immune infiltration, immune checkpoint expression and biomarkers of immune cells. In conclusion, was lowly expressed and correlated with an unfavorable prognosis in LGG. A total of six miRNAs and four lncRNAs were identified as being able to modulate in a lncRNA-miRNA-mRNA network of LGG. Furthermore, the current findings also indicated that SPTBN2 possessed anti-tumor roles by regulating tumor immune infiltration and immune checkpoint expression.
大多数成人低级别胶质瘤（LGG）最终都会随着时间的推移进展为胶质母细胞瘤。非红细胞血影蛋白β2（SPTBN2）在多种肿瘤中被检测到，并参与肿瘤的发生和转移。然而，SPTBN2在LGG中的具体作用和详细机制在很大程度上尚不清楚。本研究利用癌症基因组图谱（The Cancer Genome Atlas）和基因型-组织表达数据库（The Genotype-Tissue Expression）对LGG中SPTBN2的表达和预后进行了泛癌分析。采用蛋白质免疫印迹法检测胶质瘤组织和正常脑组织中SPTBN2的含量。随后，基于表达、预后、相关性和免疫浸润，鉴定出调控SPTBN2表达的非编码RNA（ncRNA）。最后，对与SPTBN2和预后相关的肿瘤免疫浸润情况进行了分析。SPTBN2低表达与LGG预后不良相关。观察到低SPTBN2 mRNA表达与不良临床病理特征之间存在显著相关性，包括野生型异柠檬酸脱氢酶状态（P<0.001）、1p/19q非共缺失（P<0.001）和老年患者（P=0.019）。蛋白质免疫印迹结果显示，与正常脑组织相比，LGG组织中SPTBN2的含量显著降低（P=0.0266）。包括miR-124和miR-21在内的5种微小RNA（miR/miRNA）高表达通过靶向LGG中的SPTBN2与预后不良相关。随后，观察到4种长链ncRNA（lncRNA）[反义RNA 1]通过5种miRNA对SPTBN2进行调控。此外，SPTBN2的表达与肿瘤免疫浸润、免疫检查点表达及免疫细胞生物标志物显著相关。总之，SPTBN2在LGG中低表达且与不良预后相关。共鉴定出6种miRNA和4种lncRNA能够在LGG的lncRNA-miRNA-mRNA网络中调节SPTBN2。此外，目前的研究结果还表明，SPTBN2通过调节肿瘤免疫浸润和免疫检查点表达发挥抗肿瘤作用。
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