Uribe A, Rubio C, Johansson C
Scand J Gastroenterol. 1986 Mar;21(2):246-52. doi: 10.3109/00365528609034655.
Forty Sprague Dawley rats (120 g) were divided in groups of five rats each, and 2 mg , kg-1 15(R)15-methyl-prostaglandin E2 or vehicle was administered orally, twice daily for 5 days. On the 6th day, 1 mCi . kg-1 methyl-3H-thymidine was given intraperitoneally to all rats. Groups of rats were killed at 45 min and 24 h, 72, and 120 h after the labelling. Treatments were continued until death. Samples were taken from the corpus, antrum, and jejunum and processed for autoradiography. Microscopic evaluation of the proliferative and functional compartments included cell counts and determination of the labelling index (LI) and mitotic index (MI). Prostaglandin treatment increased the number of cells in the jejunal and gastric epithelia. The DNA synthesis, evaluated from the LI and 45 min after thymidine injection, was unaffected by treatment. The clearance of label from jejunal crypts and villi was significantly slower in the prostaglandin groups. Similar observations were made in the proliferative zone of the corporal and antral epithelia. The MI was unchanged or reduced by prostaglandin treatment, the reduction being significant after 8 to 10 days' treatment. It is suggested that the trophic action of prostaglandin E2 is produced by reduction of epithelial cell losses, thereby prolonging the cell survival time. The reduced MI may be secondary to negative feedback from the hyperplastic epithelium. Trophic actions are produced by short-term treatments.
将40只体重120克的斯普拉格-道利大鼠分成每组5只的小组，每组大鼠口服2毫克/千克的15（R）-15-甲基前列腺素E2或赋形剂，每日两次，共5天。在第6天，给所有大鼠腹腔注射1毫居里/千克的甲基-3H-胸腺嘧啶核苷。在标记后45分钟、24小时、72小时和120小时处死各组大鼠。治疗持续至动物死亡。从胃体、胃窦和空肠取材并进行放射自显影处理。对增殖和功能区室的显微镜评估包括细胞计数以及标记指数（LI）和有丝分裂指数（MI）的测定。前列腺素治疗增加了空肠和胃上皮中的细胞数量。从LI和胸腺嘧啶核苷注射后45分钟评估的DNA合成不受治疗影响。前列腺素组中空肠隐窝和绒毛的标记清除明显减慢。在胃体和胃窦上皮的增殖区也有类似观察结果。前列腺素治疗使MI不变或降低，在治疗8至10天后降低显著。提示前列腺素E2的营养作用是通过减少上皮细胞丢失产生的，从而延长了细胞存活时间。MI降低可能继发于增生上皮的负反馈。营养作用由短期治疗产生。
