State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Oral Dis. 2024 May;30(4):1919-1934. doi: 10.1111/odi.14611. Epub 2023 May 8.
Serum response factor (SRF) and myocardial-associated transcription factor-A (MRTF-A) had different regulatory effects on the tumorigenesis and development in different cancers. However, the role of MRTF-A/SRF in oral squamous cell carcinoma (OSCC) remains to be determined.
CCK-8 assay, cell scratch experiment, and transwell invasion assay were conducted to investigate the effects of MRTF-A/SRF on biological behavior of OSCC cells. The expression pattern and prognostic value of MRTF-A/SRF in OSCC were analyzed based on cBioPortal website and TCGA database. Protein-protein interaction network was visualized to identify protein functions. Go and KEGG pathway analyses were performed to investigate related pathways. The effect of MRTF-A/SRF on epithelial-mesenchymal transformation (EMT) of OSCC cells was explored by western blot assay.
Overexpression of MRTF-A/SRF inhibited the proliferation, migration, and invasion of OSCC cells in vitro. High expression of SRF was related to better prognosis of OSCC patients on hard palate, alveolar ridge, and oral tongue. Besides, overexpression of MRTF-A/SRF inhibited the EMT of OSCC cells.
SRF was closely related to the prognosis of OSCC. High expression of SRF and its co-activator MRTF-A inhibited proliferation, migration, and invasion of OSCC cells in vitro, possibly via EMT suppression.
血清反应因子（SRF）和心肌相关转录因子-A（MRTF-A）在不同癌症中对肿瘤发生和发展有不同的调节作用。然而，MRTF-A/SRF 在口腔鳞状细胞癌（OSCC）中的作用仍有待确定。
通过 CCK-8 检测、细胞划痕实验和 Transwell 侵袭实验，研究了 MRTF-A/SRF 对 OSCC 细胞生物学行为的影响。基于 cBioPortal 网站和 TCGA 数据库分析了 MRTF-A/SRF 在 OSCC 中的表达模式和预后价值。可视化蛋白质-蛋白质相互作用网络以鉴定蛋白质功能。进行了 GO 和 KEGG 通路分析以研究相关通路。通过 Western blot 实验探讨了 MRTF-A/SRF 对 OSCC 细胞上皮-间充质转化（EMT）的影响。
MRTF-A/SRF 的过表达抑制了 OSCC 细胞在体外的增殖、迁移和侵袭。硬腭、牙槽嵴和口腔舌部 OSCC 患者中 SRF 的高表达与更好的预后相关。此外，MRTF-A/SRF 的过表达抑制了 OSCC 细胞的 EMT。
SRF 与 OSCC 的预后密切相关。SRF 及其共激活因子 MRTF-A 的高表达抑制了 OSCC 细胞在体外的增殖、迁移和侵袭，可能通过 EMT 抑制。
