[Overexpression of chicken Klf2 promotes transcription and inhibits adipocyte differentiation].

Overexpression of Krüppel like factor 2 (Klf2) or Klf7 inhibits adipocyte formation. However, it remains unclear whether Klf2 regulates expression in adipose tissue. In this study, oil red O staining and Western blotting were employed to study the effect of Klf2 overexpression on the differentiation of chicken preadipocytes. The results showed that Klf2 overexpression inhibited the differentiation of chicken preadipocytes induced by oleate and the expression of , while promoted expression in chicken preadipocytes. Spearman correlation analysis was used to study the correlation between the expression data of and in the adipose tissue of both human and chicken. The results showed that there was a significantly positive correlation between the expression of and in adipose tissues (r > 0.1). Luciferase reporter assay showed that overexpression of Klf2 significantly promoted the activity of chicken promoter (-241/-91, -521/-91, -1 845/-91, -2 286/-91, -1 215/-91; < 0.05). In addition, the activity of promoter (-241/-91) reporter in chicken preadipocytes was significantly positively correlated with the amount of overexpression plasmid transfected (=0.917 66, =1.074×10). Moreover, Klf2 overexpression significantly promoted the mRNA expression of in chicken preadipocytes ( < 0.05). In conclusion, upregulation of expression might be one of the pathways that Klf2 inhibits chicken adipocyte differentiation, and the sequence from -241 bp to -91 bp upstream chicken translation start site might mediate the regulation of Klf2 on transcription.